中文摘要：

构建了慢病毒载体表达MP1多肽的RFP融合蛋白（RFP-MP1）,并研究了它对人肺腺癌细胞株H1299和人骨髓瘤细胞株U2-OS增殖的影响。U2-OS和H1299细胞中RFPMP1的表达导致了RB在蛋白水平上的积累,使细胞生长受到抑制。此外,细胞流式结果发现RFP-MP1使细胞周期阻滞在G1期。进一步研究表明RFP-MP1能够阻滞RB对E2F活性的抑制。这些结果表明,11肽的MP1能够上调肿瘤细胞中RB蛋白的表达水平并且抑制其生长。

英文摘要：

A lentivirus expressing MP1 fusion protein （RFP-MP1） were generated. Its effects on the proliferation of cultured human myeloma cell line U2-OS and human lung adenocarcinoma H1299 cells by upregulating RB protein level. The expressing of MP1 in U2-OS and H1299 were studied results in an accumulation of RB protein levels leading to inhibition of cell proliferation. In addition, flow cytometry assay showed that MP1 can induce cell cycle G1 arrest. Moreover, expression of MP1 can inhibit E2F activity.