中文摘要：

为寻找简易有效的大豆叶部病害研究方法，利用大豆离体叶片，进行了相关试验。试验结果表明，大豆离体复叶培养30d，新根长达16．8～41．2cm，平均根长〉24cm，移至营养杯30d后，叶色及叶片仍保持完好。经离体叶片法和整株法接种大豆锈病病原菌后，不同品种和不同菌株表达出不同的病斑反应型；接种大豆白粉病病原菌，也同样表达出在不同品种和不同接种浓度条件下，大豆白粉病发生程度不同；两种接种方试验结果一致。本方法试验条件可控、易控，省时，省空间，因此可者虑应用于大豆叶部病害研究的各种试验。

英文摘要：

Detached leaves were used to find a simple and reliable method for studying the foliar diseases of soybean. The result showed the root generated from soybean detached leaf as long as 16.8～41.2 cm（with mean length of more than 24 cm）after culturing for 30 days,and remained green for additional 30 days in a pot with soil. It demonstrated that detached leaves produced identical or similar responses to leaves from the whole plants when inoculated with two fungal pathogens soybean rust （Phakopsora pachyrhizi Syd. ） and soybean powdery mildew（Microsphaera cliff usa）. This method could be applied for other foliar diseases study of soybean with easier controlled experiment condition and without more time and space.