以胶质芽孢杆菌(Bacillus mucilaginosus)和棘孢曲霉(Aspergillus aculeatus)分别作为细菌和真菌的代表,进行了黑云母的生物风化实验(为期50d),研究了微生物作用下黑云母中元素溶出速率和溶出顺序,并对溶解机制进行了初步探讨。利用高效液相色谱仪和电感耦合等离子体发射光谱仪分别测定了培养液中的低分子量有机酸和主要元素(Si、Al、Fe、Mg和K)的浓度。研究结果表明:胶质芽孢杆菌和棘孢曲霉均可促进黑云母的风化;棘孢曲霉的风化速率大约是胶质芽孢杆菌的65倍;真菌与细菌作用下黑云母中元素溶出顺序基本相同,即:层间→八面体晶片→四面体晶片;微生物主要通过分泌低分子量有机酸(酸化环境和络合)来促进黑云母的风化。
The differences and similarities of biotite bio-weathering between bacteria and fungi were compared using Bacillus mucilaginosus and Aspergillus aculeatus incubated for 50 days in batch reactors. Dissolution was monitored by inductively coupled plasma optical emission spectrometry (ICP-OES) analysis of elements (A1, Fe, K, Mg and Si) released into the leach solution. The low molecular weight organic acids concentrations were monitored by high performance liquid chromate graphy (HPLC). Our results showed that both Bacillus mucilaginosus and Aspergillus aculeatus accelerated the dissolution of biotite, and the dissolution rate of biotite was about 65 times in Aspergillus aculeatus experiment than that in Bacillus mucilaginous experiment. Biotite dissolves incongruently with a preferential release of the interlayer cations followed by the octahedral and subsequently the tetrahedral site. Microorganism accelerated the dissolution of biotite mainly by organic acids (acidification and complex).