中文摘要：

目的：探讨三七总皂苷（Panax notoginseng Total Saponins）对D-半乳糖致心肌细胞株H9c2细胞衰老的保护作用及其可能机制。方法：50 mmol/L D-半乳糖处理H9c2细胞建立衰老模型。不同浓度三七总皂苷（5、25、50μg/mL）预处理细胞4 h,再加入D-半乳糖继续培养24 h。β-半乳糖苷酶染色法鉴定细胞衰老程度,DCFH-DA探针检测细胞内活性氧（Reactive Oxygen species,ROS）水平,生化法检测超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量,Hochest染色分析细胞凋亡情况。结果：与正常对照组比较,D-半乳糖组β-半乳糖苷酶染色阳性细胞数目显著增多,ROS荧光强度显著上升,SOD活性降低,MDA含量增加,Hochest染色发现细胞核染色质浓缩和凝聚,并可见大量凋亡小体;与D-半乳糖组比较,不同浓度三七总皂苷组β-半乳糖苷酶染色阳性细胞数目显著减少,SOD活性显著升高,MDA含量显著降低,ROS荧光强度显著降低,细胞核染色质浓缩和凝聚以及凋亡小体均有所改善。结论：三七总皂苷可通过提高抗氧化能力和减少细胞凋亡来对抗D-半乳糖所致的H9c2细胞衰老。

英文摘要：

Objective：To investigate the protective effect of Panax notoginseng Total Saponins（ PNTS） on D-galactose-induced H9c2 cell senescence and the underlying mechanism. Methods：D-galactose was used to cause H9c2 cells senescence. Different concentrations of PNTS（5,25 and 50 μg /mL）were added into medium to protect H9c2 cells. Cell senescence was identified by senescence associated β-galactosidase. Level of reactive oxgen species（ROS）was observed according to the effect of DCFH-DA detection. The activity of superoxide dicmutase（SOD）and the content of malondialdehyde（MDA）in cells were measured by biochemical assay kits. The apoptosis of cells was tested by Hochest. Results：Compared with the control group,the number of β-galactosidase positive cells and the fluorescence intensity of ROS in the model group were markedly increased. Meanwhile,the activity of SOD was decreased whereas the content of MDA was increased. The apoptosis level assessed by the Hochest dyeing was significantly increased with chromatin concentration and condensation. Compared with the model group,the quantity of β-galactosidase in different PNTS treatment group was obviously decreased,the activity of SOD was increased and the content of MDA was reduced. The apoptosis rate in the cells treated with PNTS was improved. Conclusion：PNTS improved D-galactose-induced H9c2 cell senescence through upregulation of antioxidative ability and attenuation of cell apoptosis.