中文摘要：

目的探讨内嗅皮层注射Orexin—A对Sprague-dawley（SD）大鼠睡眠．觉醒周期以及对空间记忆的影响。方法选用健康成年雄性sD大鼠45只，体质量250—300g，采用同步记录大鼠脑电和肌电活动，观察Orexin—A及Orexinl受体阻断剂SB一334867内侧内嗅皮层微注射后大鼠睡眠-觉醒周期变化。采用T一迷宫交替选择次数，观察SB-334867内侧内嗅皮层微注射后大鼠T．迷宫选择正确率的变化。结果以人工脑脊液（ACSF）、二甲亚砜（DMSO）自身对照相比，分别给予大鼠内侧内嗅皮层双侧微注射OrexinA及SB一3348670．5p．1后3h（11：00—12：00、12：00～13：00、13：00—14：00）觉醒周期时间无影响（P〉0．05），给药后24h觉醒周期时间也无显著差异（P〉0．05）。微量注射药物后，Orexin．A（0．1nmoI／0．5txl）及SB-733467（6mmoI／0．5“1）在11：00—14：003h内对觉醒时间、NREM时间及REM时间均无影响（P〉0．05），即对3h内（1l：00—14：00）大鼠总睡眠觉醒时间无显著性差异（P〉0．05）。与DMSO组比较大鼠内侧内嗅皮层双侧微注射SB一3348670．5Ixl后，T一迷宫交替选择正确次数明显减少，正确率下降，有显著性差异（P〈0．001）。结论内侧内嗅皮层Orexin系统的支配可能参与了空间记忆，而不参与睡眠一觉醒周期调节。

英文摘要：

Objective To study the effect of Orexin-A injected into medial entorhinal cortex （MEC） of rats on sleep-wake cycle and spatial memory of SD rats. Methods Forty-five healthy adult male SD rats, weighing 250 to 300 g, were included in this study. Their cerebral and myoelectrical activities were synchronically recorded, their sleep-wake cycle and accuracy rate of alternative selection in T-maze were observed after injection of Orexin-A and SB-334867. Results Oexin-A and SB-334867 showed no significant effect on sleepwake cycle, NREM and REM sleep time of rats 3 h after they were injected into MEC of rats compared with that of ACSF and DMSO （P 〉 0.05 ）. However, the accuracy rate of alternative selection in T-maze was significantly lower after injection of SB-733467 than after injection of DMSO（P 〈0. 001 ）. Conclusion The Orexin system in MEC may be involved in spatial memory but not in sleep-wake cycle.