利用显微技术对耳鲍(HaliotisasinineLinnaeus)的胚胎发育进行了研究,同时观察到孵化后的担轮幼虫。结果表明,耳鲍胚胎发育过程与皱纹盘鲍(Haliotisdiscushannai)和杂色鲍(Haliotisdiversicolor)基本相似,胚胎发育过程分为受精期、第一极体放出期、第二极体放出期、2细胞期、4细胞期、8细胞期、16细胞期、32细胞期、桑椹期、原肠期和未孵化的担轮幼虫期。在海水质量密度11024,水温28℃的条件下,耳鲍完成胚胎发育历时约5h30min,比皱纹盘鲍(海水质量密度1.022~11023,水温22.5℃)、杂色鲍(海水质量密度1.022~1.023,水温24~26℃)分别快1.5~2.5h和0.5h。耳鲍成熟卵卵径为0.18~0.19mm,担轮幼虫具有1束顶毛和2个纤毛环带。
For promoting and improving mariculture of Haliotis asininna Linnaeus, the embryonic development of the abalone was studied using microtechnique, and the trochelminth larva was observed. The results showed that the embryonic development of H. asininna resembled those of Haliotis discus hannai and Haliotis diversicolor, and was divided into 11 periods of comprising fertilization as follows: the first polar body stage, the second polar body stage, 2-cell stage, 4-cell stage, 8-cell stage, 16-cell stage, 32-cell stage, morula stage, gastrula stage and non-hatched trochelminth larva stage. Under seawater specific gravity 1. 024 and water temperature 28℃,the embryonic development of H. asininna needed about 5 and half hours, which developed faster about 1.5 to 2.5 hours than H. discushannai (seawater specific gravity 1. 022~1. 023 and water temperature 22. 5℃), and half an hour than H. diversicolor (seawater specific gravity 1. 022-1. 023 and water temperature 24-26℃). The diameter of the matured egg was about 0.18~0.19 mm; the larva had a bundle of vertical seta and two cilia-clitellums.