中文摘要：

目的：探讨不同应变对骨髓间充质干细胞系细胞骨架形态的影响。方法：实验分为六组，Ⅰ组（静态培养）、Ⅱ组（静态培养＋细胞松驰素B）、Ⅲ组（10％应变12h）、Ⅳ组（10％应变12h＋细胞松驰素B）、Ⅴ组（10％应变24h）、Ⅵ组（10％应变24h＋细胞松驰素B）。分别对其施加10％0．5Hz的周期性应变，采用激光共聚焦显微镜技术和考马斯亮蓝染色方法对小鼠骨髓间充质干细胞系（D1细胞）细胞骨架进行形态观察、细胞F-肌动蛋白表达定量分析。结果：细胞受到不同周期性应变后，细胞的排列方向发生改变，细胞内的F-肌动蛋白排列同细胞的方向一致，随着拉伸时间的延长，F-肌动蛋白发生部分的断裂，F-肌动蛋白的荧光强度同静态组相比明显减弱（P〈0．01）；当加入细胞松驰素B后，细胞内F-肌动蛋白结构发生改变，在力的作用下，细胞内微丝断裂明显，随着拉伸时间的延长，微丝断裂更为加剧。F-肌动蛋白的荧光强度同静态组相比显著减弱（P〈0．01）。结论：不同周期性应变对细胞微丝结构产生一定的影响，微丝在细胞感应力的响应中起重要作用。

英文摘要：

Objective： To investigate the effects of different strain on cytoskeleton of mouse marrow mesenchymal stem cells in vitro. Methods： The experiment were divided into six groups, Ⅰ group was unstrained, group Ⅱ[ unstrain ＋ cytochalasin B （CB） ] , group Ⅲ （ 10 % strain for 12 h）, group Ⅳ（ 10 % strain ＋ CB for 12 h） ,group Ⅴ （ 10 % strain for 24 h） ,group Ⅵ （10 % strain ＋CB for 24 h）. 10 % 0.5 Hz cyclic strain force was applied on the mouse marrow mesenchymal stem cells at a different time,the cell was marked by doubling label technique using FITC-phalloidin and PI and Morphological was observed by confocal laser scanning microscope and BB staining. Results： the rearrangements of cell and F-actin occurred at 12 h after cyclic strain, the distribution of F-actin was destroyed by strain after 24 h. Its quantified fluorescence was less than that of the controls. The structure of F-actin were changed after CB was enterded,The cell skeleton were ruptured in strain condition, Its quantified fluorescence was significantly less than that of the controis . Conclusion ： The structure of cytoskeleton were effected by cyclic strain force, it play a important role in influence strain.