中文摘要：

在植物中,叶绿体是负责光合作用的细胞器,对叶绿体内的各种生物过程人们已经积累了很多知识,但对叶绿体蛋白质的表达还所知甚少.为了解水稻叶绿体蛋白质在正常生长发育过程中的表达情况,尝试基于抗体的水稻蛋白质组学策略.选取了10个水稻叶绿体基因,利用表达的蛋白质或合成的抗原决定簇片段制备了抗体,用Westernblotting检测了相应蛋白质在5个发育时期的根、茎、叶及穗组织中的表达.发现10个蛋白质均在叶片中表达,在根中不表达.与原初反应相关的叶绿素A/B结合蛋白1和2（CAB1和CAB2）、与电子传递相关的放氧增强蛋白1（OEE1）及与活性氧清除相关的过氧还蛋白过氧化物酶（2-CysP）和硫氧还蛋白（Trx）在茎中表达.而在卡尔文循环中发挥作用的Rubisco活化酶（RCA）、甘油醛-3-磷酸脱氢酶（GAPDH）、果糖二磷酸醛缩酶（FBPA）和景天庚酮糖-1,7-二磷酸酶（SBPase）蛋白质在茎中不表达.在穗中,这些蛋白质的表达时序不同,CAB2和2-CysP在穗发育的全程表达,CAB1和OEE1在中后期表达,而卡尔文循环中的蛋白质只在中期表达.有意思的是,卡尔文循环中的蛋白质表达模式相似,这一结果从蛋白质表达水平支持它们之间的相互衔接关系.此外,实验还揭示了可能的蛋白质修饰、二聚体及不同的转录本现象.将目标基因的表达谱与转录谱进行比较,发现二者间有一定的平行性,但也有明显的区别.以水稻叶绿体蛋白质为对象,直观并相对定量地揭示了它们的表达模式,为阐释其功能提供了信息,也为基于抗体的水稻蛋白质组学策略提供了一个初步数据.

英文摘要：

In plants, chloroplast is the key organelle for the photosynthesis, the knowledge about biological processes in chloroplast has been accumulated. However, limited information exists on the expression of chloroplast proteins. To investigate the expression profiling of rice chloroplast proteins in different growth and developmental stages and provide a pilot experiment for rice antibody-based proteomics. To address this questions, ten rice chloroplast genes were chosen and antibodies were generated using proteins expressed in E. coli or epitope peptides synthesized in vitro as immunogen, protein expression profiling were investigated by Western blotting for root, stem, leaf and panicles at five developmental stages. The results indicated that all chloroplast proteins tested were expressed in leaf, but not detectable in root. The photosynthesis primary reaction protein CAB1 and CAB2, the electron transport protein OEE1, and the ROS scavenging-related proteins 2-CysP and Trx were expressed in stem, but four carbon fixation proteins RCA, GAPDH, FBPA and SBPase, which involved in Calvin cycle, were not detected in stem. In panicle, the chloroplast proteins showed different expression patterns, CAB2 and 2-CysP were expressed at all stages during panicle growth and development, CAB1 and OEE1 were expressed at late stage, and the four proteins involved in Calvin cycle were expressed only in the middle stage. Interestingly, four proteins in Calvin cycle showed the same expression patterns, supporting their cohesive relationship. In addition, the data revealed possible clues of post-translational modification, dimer and different forms of transcripts. Comparison analysis between the profiling of gene transcription and translation revealed parallel phenomena; however, they are quite different at least in some instances. Taking together, this experiment revealed the expression patterns of rice chloroplast proteins in a direct and relative quantitative way, provided helpful information for better understanding their function a