5-Aminolevulinic 酸(翼) ,主要光敏度杀虫药剂,作为要用来控制害虫的高度有效的、环境友好杀虫剂的一种新类型吸引了增加的注意。检验翼是否有效地行动到蚂蚱, Oxya chinensis 并且阐明翼的 detoxification 机制,到翼的危险性在 O 被估计。chinensis 和二主要新陈代谢的 detoxification 酶包括谷胱甘肽 S-transferases (GST ) 和一般 esterases (EST ) 特定的活动在不同发展阶段和 O 的不同身体节被比较。chinensis 由翼和控制对待。结果证明 ALA 在不同发展阶段展出了明显的毒性到蚂蚱。在低剂量的处理(0.0597 mmol L1 ) , O 的死亡。chinensis 到达了重要水平(在第一中间形态美女,在第二中间形态美女的 61.4%o,在第三个中间形态美女的 71.4%o,和在第 4 个中间形态美女的 64.4%o 的 55.5%o。但是,没有剂量依赖者有毒的效果。从而,我们建议那翼为为控制 O 充当光敏度杀虫药剂有潜力。chinensis。用底层显示不同中间形态美女的胸和腹部由翼对待的 CDNB 和 DCNB 的 GST 活动试金证明 1.52-5.56 褶层显著地与控制相比增加了 GST 活动。为 ESTs 特定的活动试金,然而, O 之间没有重要差别。chinensis 在不同中间形态美女以内由翼和控制对待,当 -NA,-NB 和 -NA 被用作底层时。因此,由显著地增加的 GST 活动证实了的调停 GSTs 的新陈代谢的 detoxification 可能贡献在 O 由翼免于氧化损坏和氧化应力。chinensis。
5-Aminolevulinic acid (ALA), a major photosensitivity insecticide, has attracted increasing attention as a new type of highly efficient, environmental friendly pesticide to be used to control the pest. To examine whether or not ALA acts effectively to grasshopper, Oxya chinensis and elucidate the detoxification mechanism of ALA, the susceptibility to ALA was assessed in O. chinensis and two major metabolic detoxification enzymes including glutathione S-transferases (GSTs) and general esterases (ESTs)-specific activities were compared in different development stages and different body sections of O. chinensis treated by ALA and the control. The results showed that the ALA exhibited obvious toxicity to the grasshopper in different development stages. In the low-dose treatment (0.0597 mmol L-1), the mortalities of O. chinensis reached a significant level (55.5% in the 1st instar nymphs, 61.4% in the 2nd instar nymphs, 71.4% in the 3rd instar nymphs, and 64.4% in the 4th instar nymphs. But, there was no dose-dependent toxic effect. Thereby, we proposed that ALA has the potential for acting as photosensitivity insecticide for controlling O. chinensis. GSTs activity assays using CDNB and DCNB as substrates indicated that the thorax and abdomen of the different instar nymphs treated by ALA showed 1.52-5.56 fold significantly increased GSTs activities compared with the control. However, for the ESTs-specific activity assay, there was no significant difference between O. chinensis treated by ALA and the control within different instar nymphs, when a-NA, a-NB and b-NA were used as substrates. Therefore, GSTs-mediated metabolic detoxification as evidenced by significantly increased GSTs activities might contribute to protect against oxidative damage and oxidative stress by ALA in O. chinensis.