中文摘要：

目的：探讨雌激素对大鼠骨髓间充质干细胞（rBMSCs）中Periostin表达的影响。方法：取6周龄雌性SD大鼠，分为去势大鼠模型（OVX）、假手术（Sham）、OVX＋17β-雌二醇（17β-E2）组。取Sham、OVX组rBMSCs进行分离、培养，流式细胞术鉴定细胞表面分子标记物；成骨诱导各组rBMSCs的成骨分化，碱性磷酸酶（ALP）和茜素红染色检测成骨分化情况；RT—PCR检测3组rBMSCs中Periostin、Runx2、OCN、BSP的表达水平。结果：成功构建OVX；与Sham组相比，OVX组ALP及茜素红显色均较浅，OVX＋17β-E2组能加深ALP及茜素红染色。RT—PCR结果显示，OVX组Periostin、Runx2、OCN、BSP表达显著降低；OVX＋17β-E2组的Pcriostin、Runx2、OCN、BSP表达水平均明显升高。结论：雌激素可显著提高去势大鼠BMSCs中Periosfin的表达。

英文摘要：

AIM： To investigate the effects of 17 β-estradiol （ 17β-E2 ） on the expression of periostin in rBMSCs of ovariectimized rats. METHODS： Ovariectomy （OVX） was performed to establish osteoporosis in 6 -w SD rats. rBMSCs were separated and cultured from Sham, OVX, and OVX ＋ 17β-estradiol treated rats, and then cell surface markers were indentified by flow cytometry. After osteoblastic induction, esteoblastic differentiation was evalua- ted by ALP activity and alizarin red staining. The expression level of periestin, Runx2, OCN and BSP was determined by RT - PCR. RFULTS： ALP expression and mineralization nodules were evidently observed in sham group, and significantly decreased in OVX group. 17β- E2 treatment increased the ALP expression and mineralization nodule formation. RT-PCR results showed that the expression of periostin, Rnx2, OCN and BSP decreased significantly in OVX rats. 17β- E2 treatment significantly increased the expression of periestin, Runx2, OCN and BSP （ P 〈0.05）. CON- CLUSION： 17 β-estradiol can enhance the expression of Periestin in BMSCs of ovaxiectimized rats.