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褐煤中天然产甲烷菌富集培养与生物气产出模拟

ISSN号：1006-7493
期刊名称：《高校地质学报》
时间：0
分类：P618.11[天文地球—矿床学;天文地球—地质学]
作者机构：[1]中国矿业大学资源与地球科学学院,徐州221116, [2]煤层气资源与成藏过程教育部重点实验室,徐州221116, [3]云南省煤田地质局,昆明650034
相关基金：国家自然科学基金重点项目（40730422）资助

关键词：褐煤, 厌氧细菌, 产甲烷菌, 生物气, 模拟实验, brown coal, anaerobic bacteria, methanogen, biogenetic gas, simulation experiment

中文摘要：

采用厌氧培养方法,在云南省昭通褐煤样品中成功地培养富集了活性厌氧细菌,并进一步开展了为期60天的生物气生成模拟实验,分析了生物气的生成规律、物质组成和成因机制。结果揭示：3件褐煤样品中均有活性厌氧细菌存在,以纤维素分解菌为主,活性硫酸盐还原菌极其微少,说明昭通盆地褐煤层具有较强还原性的生化环境,有利于产甲烷菌的繁盛;生物气的生成经历了生气量缓慢增长、显著增高、趋于减缓三个阶段,表明产甲烷菌在经历了第一阶段缓慢繁殖后,其数量和活性在第二阶段达到较高水平;3件煤样生气量和生气历程存在一定差别的原因,可能在于原煤中活性产甲烷菌数量以及显微组分组成方面存在差异,褐煤生物气生成过程至少存在两个以上的生气高峰阶段。模拟实验中产生的生物气几乎全部由CH4和CO2组成,且CH4占主要部分,几乎没有检测到重烃气。甲烷气体δ13C1和δD的平均值均处于生物甲烷碳、氢同位素组成的正常分布范围。

英文摘要：

Using the method of anaerobic glove chamber culture, active anaerobic bacteria were successfully enriched and cultivated in brown coal samples collected from Zhaotong Basin, Yunnan Province. Simulation experiments of biogenic gas for 60 days were further conducted to analyze the generation regularities, substance compositions and genetic mechanism of biogenic gas. The results showed that in all three brown coal samples there existed active anaerobic bacteria, among which cellulose decomposition bacteria were in majority and sulfate-reducing bacteria were very few, which means that the biochemical circumstances in the brown coal-seams of Zhaotong Basin were reductive and favourable for the growth of methanogen. The biogenic gas generation experienced through three stages： the very slow growing stage, the evidently increasing stage and the slowing down again stage, which indicated that methanogen had high density and activity in the second stage after the slow reproduction first stage. It is considered that the differences in gas generation amount and processes among three brown coal samples were probably resulted from the initial quantities of the active methanogen and maceral composition in the coal. It is proposed that there were at least two peaks of biogenic gas generation from the brown coal. Stimulated biogenic gas was mostly composed of CH4 and CO2, in which CH4 was predominant and heavy hydrocarbons were hardly detected. All the mean values of δ13C1 and δD in the methane produced from three coal samples were found to be in the normal distribution ranges compared to those of biogenic methane.

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