中文摘要：

利用Bac—to—Bac载体系统，构建了含有东亚钳蝎Buthusmartensii Karsch昆虫特异性神经毒素基因（BmK IT）和杆状病毒组织蛋白酶基因（vcath）的重组杆状病毒AcMNPV—BmKIT-vcath，使这两个基因分别位于p10基因启动子（Pp10）和多角体基因启动子（Pph）的控制下高效表达．抗棉铃虫毒力分析表明，该重组病毒的LC50值为28829．5pfu／mL，比野生型病毒的LC50值（33114．5pfu／mL）降低13％；重组病毒的LT50值为3．5d，而野生型的LT50值为4．1d，提高了14．6％，表明该重组病毒的活性比野生型病毒有明显的提高．

英文摘要：

Recombinant baculovirus AcMNPV-BmK IT-veath was constructed using Bac to Bac expression system. The insect neurotoxin gene BmK IT and baculovirus vcath gene were constructed into the genome of AcMNPV,under the control of strong promoter pl0 and polh respectively. Toxicity show the median lethal concentration （LC50） of recombinant and wild viruses were 28 829.5 （pfu/mL） and 33 114.5 （pfu/mL） respectively. The LC50 value of recombinant virus was lower 13% than wild type virus. At the concentration of 2×106 pfu/mL,the median lethal time （LT50） of two viruses were for 3.5 d and 4.1 d respectively. The LT50 of recombinant virus had a decrease of 14.6 % compared with wild type one. The results showed recombinant virus improved insecticidal potency and killing rate.