中文摘要：

以聚乳酸/聚羟基丁酸戊酸共聚酯（PLA/PHBV）作为填充床反应器的碳源和生物膜载体,对受硝酸盐污染的水进行生物反硝化脱氮.采用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）技术分析了PLA/PHBV表面生物膜中微生物群落的结构和动态变化.结果表明,反应器运行初期,生物膜中微生物多样性下降.当反应器稳定运行时,DGGE图谱特征条带的香农威尔指数和辛普森指数均变化不大,微生物群落结构保持相对稳定.DGGE图谱特征条带的16S rDNA序列分析及扫描电镜分析的结果表明,生物膜中的主要微生物均为革兰氏阴性杆菌,包括Diaphorobacter、Acidovorax、Rubrivivax、Azospira、Thermomonas和Devosia,它们分别属于变形菌门（Proteobacteria）的α-,β-和γ-变形菌纲,其中Diaphorobacter为反应器稳定运行期生物膜中丰度最高的菌群.

英文摘要：

Polylactic acid/Poly（3-Hydroxybutyrate-co-3-Hydroxyvalerate）（PLA/PHBV） granules were used as both carbon source and biofilm carrier for nitrate removal from the contaminated water.The polymerase chain reaction-denature gradient gel electrophoresis（PCR-DGGE） was used to investigate the dynamic change of microbial community in the biofilm coating onto PLA/PHBV granules.The results showed that a decrease in microbial diversity was observed at the initial stage of reactor operation.Shannon-Wiener and Simpson diversity indexes of microbial community in the biofilm slightly changed during the stable period of the reactor running.The major microorganisms in the biofilm were Gram-negative rod bacteria including the genera of Diaphorobacter,Acidovorax,Rubrivivax,Azospira,Thermomonas and Devosia,and all of them belonged to α-,β-and γ-Proteobacteria,according to the SEM photo of biofilm and 16S rDNA sequences of the DGGE profile.Moreover,the abundance of Diaphorobacter-like genera was the highest in the solid-phase-denitrification biofilm.