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中文摘要：
目的构建和表达人类疱疹病毒8型（HHV-8）K8．1基因真核表达载体。方法聚合酶链反应（PCR）法从HHV名感染细胞BCBL-1细胞总DNA中扩增HHV名K8．1全长基因；克隆入真核表达载体pcDNA3．1（＋）；将重组K8．1＋pcDNA3．1真核表达载体转染人胚肾细胞293细胞，提取总RNA，反转录PCR（RT-PCR）检测重组K8．1＋pcDNA3．1真核表达载体在293细胞中的mRNA。结果经测序，克隆的K8．1基因序列与GenBank中登记的K8．1基因100％同源；RT-PCR法检测在约370bp位置出现条带，与预期的369bp大小一致。结论HHV-8K8．1基因真核表达载体构建成功，并可在293细胞中表达。
英文摘要：
Objective To construct the recombinant of eukaryotic expression vector containing K8.1 gene from human herpesvirus 8 （HHV-8） and explore its expression in eukaryotie cell. Methods The full-length gene （HHV-8 K8.1 ） was amplified from HHV-8 infected BCBL-1 cells with polymerase chain reaction（PCR）. K8.1 DNA fragments were cloned into eukaryotic vector[ peDNA3.1 （ ＋ ） vector]. 293 cell was transfected with recombinant of K8.1 and pcDNA3.1 （ ＋ ） vector, and total RNA was extracted, mRNA of the recombinant of K8.1 and pcDNA3.1 （ ＋ ） vector in 293 cell was determined by reverse transcription-PCR （RT-PCR）. Results The sequence of cloned K8.1 gene was 100% homology with K8.1 gene registered in GenBank. A band about 370 bp as expecting（369 bp） was visible when total RNA of 293 cells was detected with RT-PCR. Conclusion The eukaryotic expression vector of HHV-8 K8.1 has been constructed, and can be expressed in 293 cell.
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