中文摘要：

目的：探索新生大鼠脑皮质少突胶质Ⅱ型星形祖细胞（OP）的纯化及鉴定。方法：分离、培养新生大鼠皮质混合胶质细胞,采用改良的振荡分离纯化法获取OP细胞,并进行诱导分化和免疫组化鉴定。结果：用该方法获取的OP细胞A2B5和Nestin免疫染色为双阳性,细胞纯度可达90%以上,且具有分化为星形胶质细胞和少突胶质细胞的双分化潜能。结论：低速振荡结合2次接种可纯化得到高纯度的OP细胞,是一种简单、高效的OP细胞的纯化方法。

英文摘要：

Aim：To explore the method of culture and purification of oligodendrocyte-type-2-astrocyte progenitor cells derived from cortex of new-born rats and identification.Methods：Mixed glial cells derived from cortex of neonate rats were cultured and oligodendrocyte-type-2-astrocyte progenitor cells were purified by modified method of shaking isolation.The purified cells were then induced to differentiation and identified by immunochemical staining.Results：oligodendrocyte-type-2-astrocyte progenitor cells exhibit double positive staining of A2B5 and Nestin,which have the potential ability to differentiate into astrocytes and oligodendrocytes.The purification of isolated cells can reach over 90%.Conclusion：High purification oligodendrocyte-type-2-astrocyte progenitor cells can be acquired by low rate shaking combined with secondary plated method,so it is a simple and high effective method for the purification of oligodendrocyte-type-2-astrocyte progenitor cells.