中文摘要：

瞄准：决定声音的刺猬(嘘) 的角色在颜色的小径通过嘘小径相关的分子，嘘， Ptch1，交往刺猬的蛋白质(臀部) ， Gli1， Gli3 和 PDGFRalpha 的表示的分析的表面的腺癌。方法：在 25 颜色的嘘的表示表面的腺癌被 RT-PCR，原位杂交和免疫组织化学检测。Ptch1 的表示被原位杂交和免疫组织化学观察。臀部， Gli1， Gli3 和 PDGFRalpha 的表示被原位杂交分析。结果：cytokeratin AE1/AE3 的表示在颜色的细胞质被观察表面的地窟。表明小径的 Hh 的成员在颜色被表示表面的上皮。当 Ptch1，臀部和 Gli1 的表示主要在腺癌的恶意的地窟被检测时，嘘在发育异常的上皮细胞的细胞质被表示。相反， PDGFRalpha 在基质在异常地窟并且中等高度被表示。Gli3 的表示不能在颜色被检测表面的腺癌。结论：这些数据建议 Shh-Ptch1-Gli1 发信号小径可以在颜色的前进起一个作用表面的肿瘤。

英文摘要：

AIM： To determine the role of Sonic hedgehog （Shh） pathway in colorectal adenocarcinomas through analysis of the expression of Shh pathway-related molecules, Shh, Ptchl, hedgehog-interacting protein （Hip）, Gil1, Gli3 and PDGFRα. METHODS： Expression of Shh in 25 colorectal adenocarcinomas was detected by RT-PCR,in situ hybridization and immunohistochemistry. Expression of Ptchl was observed by in situ hybridization and immunohistochemistry. Expression of Hip, Gil1, Gli3 and PDGFRα was analyzed by in situ hybridization. RESULTS： Expression of cytokeratin AE1/AE3 was observed in the cytoplasm of colorectal crypts. Members of the Hh signaling pathway were expressed in colorectal epithelium. Shh was expressed in cytoplasm of dysplastic epithelial cells, while expression of Ptchl, Hip and Gill were mainly detected in the malignant crypts of adenocarcinomas. In contrast, PDGFRα was expressed highly in aberrant crypts and moderately in the stroma. Expression of Gli3 could not be detected in colorectal adenocarcinomas. CONCLUSION： These data suggest that Shh-Ptchl-Gli1 signaling pathway may play a role in the progression of colorectal tumor.