中文摘要：

目的探讨多亮氨酸重复区免疫球蛋白样蛋白1（LRIG1）与胶质瘤细胞化疗敏感性的相关性。方法替莫唑胺诱导构建多药耐药细胞株U251／TR，细胞计数试剂盒（CCK-8）检测该细胞株的多药耐药性，逆转录-聚合酶链反应（RT—PCR）法检测LRIG1在U251和U251／TR中的表达变化。转染LRIG1质粒体进入多药耐药细胞株，检测其对化疗药物敏感性变化。结果成功构建多药耐药细胞株U251／TR，替莫唑胺、阿霉素、环磷酰胺、依托泊苷、硫酸长春新碱对U251的抑制率分别为：（19．30±0．04）％、（39．90±0．13）％、（28．10±0．09）％、（66．20±0．02）％、（26．30±0．11）％。而对U251／TR的抑制率分别为：（3．20±0．03）％、（15．30±0．09）％、（4．10±0．03）％、（45．60±0．10）％、（10．80±0．04）％，两者差异有统计学意义（P〈0．05）。其LRIG1的表达明显降低，将LRIG1质粒体转入耐药细胞株后，TMZ、VP16、硫酸长春新碱、环磷酰胺、阿霉素对空白组（耐药细胞株）的抑制率为（2．60±0．02）％、（3．30±0．02）％、（9．50±0．06）％、（2．20±0．05）％、（2．90±0．03）％，而对转染组的抑制率为（19．10±0．34）％、（38．20±0．53）％、（29．10±0．25）％、（15．20±0．55）％、（17．40±0．41）％，耐药性被逆转，差异有统计学意义（P〈0．05）。结论LRIG1与肿瘤的化疗敏感性相关，LRIG1可能与肿瘤的多药耐药有关。

英文摘要：

Objective To investigate the relationship between leucinc-rich repeats and immuno- globulin-like domains 1 （ LRIG1 ） with chemotherapy sensitivity in glioma cells. Methods Temozolomide （TMZ） resistant cell line was constructed and the expression of LRIG1 was detected by using reverse tran- scription-polymerase chain reaction （RT-PCR）. The multidrug resistance was measured by using cell count- ing Kit-8 （ CCK-8 ）. LRIG1 plasmid was transfected into the multidrug resistant cells, and chemotherapy sen- sitivity was tested. Results Multidrug resistant cell line was constructed and the cell line had resistance to temozolomide, adriamycin, cyclophosphamide, etoposide and vincristine sulfate. The inhibition rate of these drugs used to U251 cell line was as follows ： （ 19. 30 ± 0. 04 ） %, （ 39. 90 ± 0. 13 ） %, （ 28. 10 ± 0. 09 ） %, （ 66. 20 ± 0. 02） %, （26. 30 ± 0. 11 ） %, and that to U251/TR was as follows ： （ 3.20 ± 0. 03 ） %, （ 15.30 ± 0. 09 ） %, （4. 10 ± 0. 03 ） %, （ 45.60 ± 0. 10 ） %, （ 10. 80 ± 0. 04 ） %. The difference was statistically signif- icant between U251 and U251/TR. （P 〈0. 05 ）. The expression of LRIG1 was decreased in U251/TR com- pared to U251 cells, and the resistance was abolished when plasmid of LRIG1 was transfected into the multi- drug resistant cells. The inhibition rate of TMZ, VP16, vincristine, cyclophosphamide and doxorubicin used to bland group （ resistant cell line） was （2, 60 ± 0. 02 ） % , （ 3.30 ± 0. 02） % , （ 9. 50 ± 0. 06 ） % , （ 2. 20 ± 0. 05）% and （2. 90±0. 03）%, and that of these drugs used to transfected group was （19. 10 ± 0. 34）%, （38.20±0.53）%, （29. 10 ±0.25）%, （15.20 ±0.55）%, and （17.40 ±0.41）% respectively. The difference was statistically significant （P 〈 0. 05）. Conclusion The expression level of LRIG1 was associ- ated with the chemotherapy sensitivity in astrocytoma. LRIG1 was associated with the multidrug resistance of glioma.