中文摘要：

目的：通过甲基化特异性PCR研究比较女性散发性基底细胞型乳腺癌和非基底细胞型乳腺癌中ERα基因启动子区甲基化情况。方法：筛选基底细胞型乳腺癌，用甲基化特异性PCR研究60例散发性基底细胞型乳腺癌及108例非基底细胞型乳腺癌的ERα启动子甲基化情况，比较二者ERα基因启动子区甲基化情况。结果：女性散发性基底细胞型乳腺癌组织中ERα基因启动子甲基化发生率为80.0%（48/60），高于其在女性散发性非基底细胞型乳腺癌ERα基因启动子甲基化率43.5%（47/108）（χ2=20.89,P＜0.01），二者在ER1与ER5区域的甲基化无统计学差异，但在ER3与ER4区域的甲基化差异有统计学意义。结论：HT5”SS〗女性散发性基底细胞型乳腺癌组织中ERα基因启动子甲基化发生率明显高于非基底细胞型女性乳腺癌，且基底细胞型乳腺癌ER3与ER4区域的甲基化高于非基底细胞型乳腺癌，其可能与基底细胞型乳腺癌发生发展有关。

英文摘要：

Objective： To discuss the promoter methylation of estrogen receptor - alpha in sporadic basal - like and non basal - like breast cancer by methylation - specific polymerase chain reaction. Methods： To screen female sporadic basal - like breast cancer,and the methylation of ERα promoter was investigated in genomic DNA of 60 sporadic basal - like breast cancer and 108 cases of non basal - like breast cancer as control by methylation - specific polymerase chain reaction. Results：ERα methylation was observed in 48 of 60 （80.0%） sporadic basal -like breast cancer, which was significant higher than in sporadic non basal - like breast cancer（47/108,43.5% ） （ P 〈0.01 ）. There was no significancant difference in the methylation status of ER1 region and ER5 region between sporadic basal - like breast cancer and sporadic non basal - like breast cancer. The methylation status of ER3 region and ER4 region in sporadic basal - like breast cancer was higher than in sporadic non basal - like breast cancer. Conclusion： The ERα methylation status in basal - like breast cancer was significantly higher than in sporadic non basal - like breast caneer. The methylation status of ER3 region and ER4 region in sporadic basal - like breast cancer was higher than in sporadic non basal - like breast cancer, maybe it plays an important role in basal - like breast cancer pathogenesis.