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中文摘要：
为提高籼碎米蛋白质碱法提取效率，在Min—RunResIV析因设计和爬陡坡试验的基础上，利用二次旋转中心组合响应面优化方法研究碱液浓度、籼碎米粒度、固液比对大米蛋白质提取的影响规律，并优化其提取工艺条件。结果表明，籼碎米蛋白质最优碱法提取工艺为碱液浓度0．09mol／L，籼碎米粒度80目，固液比1：13．5（g／mL），碱提温度50℃。碱提时间120min。该条件下蛋白质提取率为（82．25±2．53）％，大米蛋白质产物纯度为（91．68±1．26）％。
英文摘要：
To improve the efficiency of protein extraction from broken long-grain rice, a quadratic rotatable central composite response surface methodology （qrCCD-RSM） was utilized to investigate the affecting mechanism of alkali concentrations, broken long-grain rice particle sizes and solid liquid ratios （SLR）, based on the results of Min-Run Res IV factorial design and steep accent experiments （SAE）. The optimal extraction condition was obtained, i.e. 80 mesh of broken long-grain rice was used to extrat proteins, soluting in 0.09 mol/L of NaOH buffer, with the SLR 1 g ： 13.5 mL at 50℃ for 120 min. Under this optimized condition, the protein extraction rate and its purity was found to be （82.25±2.53）% and （91.68±1.26） %, respectively.
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