中文摘要：

用50L密闭式吊袋光生物反应器,采用连续培养模式,对6株微拟球藻（Nannochloropsis oceanica）进行产油性能的中试筛选。从藻种的比生长速率、总脂含量、油脂产率,甘油三酯（TAG）含量和脂肪酸组成等方面评价藻种的产油性能。经过10d的连续培养,筛选到2株生长速率快、油脂产率高的优良能源微藻藻株：3-25和75B1,它们的总脂含量分别达到细胞干重的33.49%和29.36%;油脂产率分别为10.04和8.07mg·（L·d）^-1;它们的C16与C18之和分别占到了总脂肪酸含量的72.71%和68.05%,且以饱和脂肪酸C16∶0及单不饱和脂肪酸C16∶1为主,适合生物柴油的生产。尽管藻株4-38的油脂产率较低,但其甘油三酯含量较高,C16和C18之和高达76.32%,其脂肪酸组成也非常适合生物柴油的生产。研究结果表明,3-25、75B1和4-38这3株微拟球藻有望作为生产生物柴油的候选藻株。

英文摘要：

Renewable energy as microalgal biodiesel has raised broad interest;it is characterized by higher lipid productivity per ground area than oleaginous agricultural crops and does not compete for arable land or bio-diversifies natural landscapes.Unfortunately,agreat deal of developments must occur before such product reaches the marketplace.One of the key bottlenecks is the high cost of microalgal cultivation.For the successful commercialization of microalgae-based fuels,it is crucial to minimize culture cost and breed oil-rich microalgae.Therefore,it is imperative that the strain selected for large-scale cultivation produces the appropriate lipids suitable for biodiesel production in pilot scale.Selection criteria should be based on a number of factors including growth rate,lipid quantity and quality,strong adaptability to the change in environment and determination of preferred nutrients and nutrient uptake rate.Nannochloropsis have proved to be suitable as raw materials for commercial production due to their high biomass and oil content.In this study,six Nannochloropsis oceanica strains were screened for their oil productivity by continuous cultivation mode in pilot scale with 50 Linclosed punching bag-light bioreactors.Oil-producing characteristics were evaluated from several aspects such as specific growth rate,content of total lipids,oil productivity,triglyceride content and fatty acid composition.Two excellent strains（3-25 and 75B1）with rapid growth and high oil productivity were obtained by continuous cultivation for ten days.The content of total lipids and oil productivity of the two strains were 33.49% and 29.36%,and 10.04 mg·L^-1·d^-1 and8.07mg·L^-1·d^-1,respectively.The C16 and C18series contents of the two algal strains accounted for72.71%to 68.05%,respectively,and were found to consist largely of the saturated fatty acid（SAFA）palmitic acid（C16∶0）and the monounsaturated fatty acid（MUFA）palmitoleic acid（C16∶1）,which are ideal for biodiesel production.Strain 4-38 performed bette