中文摘要：

目的 研究安宫牛黄丸对脑缺血再灌注损伤和脑外伤大鼠的保护作用。方法 成年雄性SD大鼠连续7 d灌胃给予安宫牛黄丸,灌胃第4天线栓法致脑缺血1 h再灌注72 h后检测大鼠行为学、脑梗死百分比及脑组织炎症因子肿瘤坏死因子α（TNF-α）、白介素-1β（IL-1β）和诱导型一氧化氮合酶（i NOS）mRNA的表达。此外,大鼠连续7 d灌胃给予安宫牛黄丸后,采用重物打击法诱导大鼠脑外伤模型,24 h后检测大鼠行为学改变并通过real time RT-PCR检测脑内炎症因子TNF-α、IL-1β和i NOS mRNA的表达。结果 与脑缺血再灌注损伤模型组比较,安宫牛黄丸（1.5 g/kg）能够改善模型大鼠行为学改变,减少大鼠脑梗死面积,下调脑组织内TNF-α、IL-1β和i NOS mRNA的表达;与脑外伤模型组比较,安宫牛黄丸（1.5 g/kg）可以改善大鼠行为学改变,下调脑组织内TNF-α、IL-1β和i NOS mRNA的表达。结论 安宫牛黄丸对大鼠脑缺血再灌注损伤和脑外伤有保护作用。

英文摘要：

Objective To study the effects of Angongniuhuang Wan （AGNHW） on cerebral ischemia -reperfu- sion and traumatic brain injury in rats. Methods Adult Sprague - Dawley （SD） rats were orally given AGNHW for consecutive 7 d. Brain ischemia was induced by middle cerebral artery occlusion （MCAO） through the suture - occluded method for 1 h followed by reperfusion for 72 h at the 4th day of AGNHW administration. The behav- ior,brain infarction areas and inflammatory factors mRNA expressions were tested. Moreover, rat traumatic brain injury was induced by dropping a 20 g weight from 20 mm heights after orally administration of AGNHW for con- secutive 7 d. Twenty - four h later, the behavior was observed and rat brain inflammatory factors mRNA expres- sions were examined through real - time RT - PCR. Results In ischemia - reperfusion injury model, AGNHW （1.5 g/kg） was effective in improving the behavioral abnormality and reduced the infarction rate and suppressed the mRNA expressions of tumor necrosis factor （ TNF - a）, interleukin - 1/3 （ IL - 1/3） and inducible nitric oxide synthase （iNOS）. In traumatic brain injury model, AGNHW （1.5 g/kg） attenuated rat behavior abnormality and inhibited TNF - eL, IL - 1 I~ and iNOS mRNA expressions. Conclusion AGNHW is effective against cerebral is- chemia- renerfilsion and traumatic brain injury in rats.