中文摘要：

目的 构建一种简单可靠的同期心脏联合皮瓣移植小鼠模型,探讨复合组织与内脏器官联合移植后可能存在的相互影响.方法 取供体C57小鼠心脏及腹股沟皮瓣移植至受体C57小鼠颈部.获取供体心脏血管蒂主动脉时,保留其右侧颈总动脉分支,供体心脏主动脉与肺动脉分别与受体C57小鼠右侧颈总动脉和颈外静脉连接;获取供体腹股沟皮瓣的营养血管分别与供体心脏的右侧颈总动脉及受体左侧颈总静脉相连,即将供体颈总动脉作为血管桥,连接供体心脏与皮瓣.术后每天观察、记录皮瓣与心脏的存活情况,最后行组织病理学检查.结果 同系移植物存活时间大于100d.平均手术时间为（193.6±7.8） min,其中心脏热缺血时间为（14.4±1.7） min,皮瓣热缺血时间为（28.0±4.2） min.术后第100天,组织学检查显示,心肌、皮瓣组织细胞完好,无炎症细胞浸润.结论 我们成功地构建了一种简便可靠的同期小鼠心脏联合皮瓣移植模型,可用于复合组织与内脏器官联合移植的相关实验研究.

英文摘要：

Objective We aimed to develop a model of concomitant heart and composite tissue transplant in mice,which could be used to compare the possible surgical results.Methods Isograft transplants were performed among C57BL/6 mice.The donor＇s heart was harvested with the right common carotid artery,then the aorta and pulmonary artery were anastomosed to the recipient＇s right common carotid artery and external jugular vein,and the donor＇s abdominal flap vessels were connected to the donor＇s right common carotid artery and recipient＇s left external jugular vein,respectively.Heart viability was assessed by daily palpation,and the skin flap was observed every day.Finally,the grafts were assessed histologically.Results Indefinite allograft survival （〉100 days） was achieved in the isograft transplant group.The mean total operative time was （193.6 ± 7.8） minutes with the average warm ischemia time of heart and skin flap was （14.4 ± 1.7） minutes and （28.0 ± 4.2） minutes,respectively.Histologic examination displayed viable grafts without necrosis or fibrosis.Conclusion We succeeded in developing a technically feasible and reliable model of simultaneous heart and composite tissue transplant in mice which may be used in the research of mutual immunologic influence and split rejection between composite tissue and solid organ.