中文摘要：

目的研究SOX4基因在前列腺癌患者中的表达；探讨二烯丙三硫（DATS）对SOX4表达阳性前列腺癌细胞的生物学作用及对SOX4表达的调节作用。方法采用免疫组织化学PV-9000二步法检测141例前列腺癌患者SOX4的表达，观察其与临床病理指标和预后的关联。采用不同浓度（0、20、40、60μmol／L）DATS处理前列腺癌Vcap细胞株，细胞增殖活性MTs法测定Vcap细胞的增殖能力；细胞体外侵袭实验检测40μmol／LDATs对Vcap细胞侵袭能力的作用；实时定量PCR（Real—timePCR）检测40μmol／LDATS作用后，Vcap细胞中SOX4mRNA的表达水平变化。结果SOX4蛋白在21％（28／135）的前列腺癌患者中过表达，与Gleason评分、远处转移呈正相关（P〈0．05）。SOX4是影响患者生存的独立预后因素。体外实验显示，20～60μmol／LDATs对前列腺癌细胞株Vcap的生长有较明显的抑制作用，且呈浓度和时间依赖性。40μmol／LDATs明显抑制Vcap细胞的侵袭能力，并在mRNA水平上下调SOX4的表达。结论SOX4的过表达提示前列腺癌患者预后不良。DATS抑制前列腺癌Vcap细胞的增殖和侵袭能力，并在mRNA水平上显著下调SOX4的表达。

英文摘要：

Objective To characterize the expression of SOX4 in prostate cancer patients and investigate the effect of di- allyl trisulfide （DATS） on proliferation and invasion of SOX4-positive prostate cancer cells and its role in regulation of SOX4. Methods The PV9000 two-step immunohistochemical method was utilized to examine the expression of SOX4 protein in 141 prostate cancer patients. Correlation of SOX4 expression with clinicopathological parameters and progno- sis were also analyzed. Prostate cancer cell line Vcap, which demonstrated overexpression of SOX4, was treated with various concentrations of DATS （0,20,40,60 μmol/L）, followed by MTS assay to determine its cellular proliferation; Matrigel invasion assay was used to assess the effect of 40 p.mol/L DATS on the invasive capacity of Vcap cells. Real time-PCR was performed to detect mRNA expression level of SOX4 by 40μmol/L DATS treatment. Results Overex- pression of SOX4 protein was preset in 21% （28/135） of prostate cancer patients, and significantly correlated with high Gleason score and the presence of distant metastasis （ P 〈 0.05 ）. SOX4 was an independent prognostic factor for prostate