中文摘要：

目的探讨3岁以下反复喘息患儿外周血血清类黏蛋白1样蛋白3（ORMDL3）基因在不同临床表型中的表达水平及其临床意义。方法选取2010年9月至2012年9月在南京医科大学第一附属医院就诊的3岁以下反复喘息患儿25例，分为非特应性体质的喘息A组（14例）和特应性体质的喘息B组（11例）；健康对照组无任何变应性疾病病史，包括药物及食物过敏、变应性鼻炎、湿疹，共24例，采用反转录PCR法检测外周血ORMDL3基因mRNA表达水平，并分析各组病例临床特点。结果喘息A组和喘息B组ORMDL3基因表达水平高于健康对照组（t=10．29、5．73，P〈0．01），喘息B组ORMDL3基因水平高于喘息A组，差异有统计学意义（t=14．12，P〈0．01）。喘息组中发病率存在性别差异，男〉女；喘息发作存在季节差异，冬季和春季高发。结论ORMDL3基因的表达水平与〈3岁患儿的反复喘息密切相关，在特应性体质患儿更为明显，提示ORMDL3在反复喘息发病机制中起一定作用。

英文摘要：

Objective To study the mRNA expression levels and clinical significance of orosomucoid l-like protein 3 （ORMDIA） gene in the peripheral blood of recurrent wheeze children under 3 years old. Methods Peripheral blood specimens of 25 recurrent wheeze children including 14 non-atopy patients （group A） and 11 atopy patients （group B） that were registered in the First Affiliated Hospital of Nanjing Medical University, from Sep. 2010 to Sep. 2012 were enrolled based on the inclusion criteria and 24 non-allergic controls （the children with food allergy, drug al- lergy or ectema was excepted）. The mRNA expression levels of ORMDL3 gene were detected by reverse transcription （RT）-PCR and clinical features were analyzed. Results The expression levels of ORMDL3 were up-regulated in the peripheral blood specimens of group B compared with group A（t = 14. 12,P 〈 0. 01 ）. Compared with peripheral blood specimens from normal subjects, mRNA expression of ORMDL3 were significantly increased in recurrent wheeze children（t = 10. 29,5.73 ,P 〈0. 01 ）. The incidence of wheeze groups exist gender differences, male 〉 female. Wheeze usually with a high incidence in winter and spring. Conclusions The increase of ORMDL3 gene expression levels were correlated with recurrent wheeze under 3 years old especially in atopy group and may be involved in the pathogenesis of recurrent wheeze.