中文摘要：

目的：研究F10基因对转录因子NF-κB、AP1活性的影响。方法：质脂体共转染F10基因和报道质粒NF-κB-Luc、AP1-Luc，通过测定荧光素酶的活性以确定F10基因对NF-κB和AP1转录活性的影响；提取各实验组不同时点细胞核蛋白，用电泳迁移率改变实验（electrophoretic mobility shift assay，EMSA）检测AP1、NF-κB和DNA的结合活性。结果：F10基因瞬间转染A549细胞48hNF-κB-Luc和AP1-Luc荧光素酶的活性较其对照组分别下降0．3和上升2倍、AP1和NF-κB与DNA的结合活性分别上升23％和下降49％。结论：F10基因上调AP1活性、下调NF-κB的活性。

英文摘要：

AIM： To investigate the influence of F10 on the activities of transcription factor NF-κB and AP1 in A549 cells. METHODS： The luciferase report plasmids of NF-κB - Luc, AP1 - Luc and F10 gene were introduced into A549 ceils and the luciferase activity was detected. The DNA binding activities of AP1 and NF-κB in the ceils were measured by the electrophoretic mobility shift assay （EMSA）. RESULTS： The luciferase activity in F10^＋ transfection group decreased 30% and increased 2 - fold respectively 48 h after transfected with the luciferase report plasmid of NF-κB - Luc and AP1 - Luc in A549 cells. The DNA binding activity decreased 49% and increased 23% ,respectively. CONCLUSION： F10 gene up -regulates the transcription activity of AP1 and down -regulates the NF-κB in A549 cells.