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盐胁迫下ABA预浸种对向日葵幼苗的缓解效应
  • ISSN号:1000-6850
  • 期刊名称:《中国农学通报》
  • 时间:0
  • 分类:Q945.78[生物学—植物学]
  • 作者机构:[1]东北师范大学生命科学学院,长春130024, [2]空军航空大学航空救生系,长春130022
  • 相关基金:国家自然科学基金生物学基地项目(J0830627-2);国家自然科学基金资助项目“基于生态位动态的草本-木本植物竞争共存机制及生态耦合效应研究”(41101523)
中文摘要:

浸种预处理可以缓解外界环境胁迫对植物的影响。以向日葵为材料,研究10 mg/L脱落酸(ABA)预浸种对盐胁迫下(150 mmol/L NaCl)向日葵幼苗相应生理参数的影响。实验结果表明:与正常生长的向日葵幼苗(A0)相比,150 mmol/L NaCl胁迫(A1)处理使得幼苗中叶绿素a、叶绿素b、类胡萝卜素、色素总含量分别降低了7.8%、3.6%、0.5%、10.9%;经ABA浸种处理后,各种光合色素含量不同程度提高;比较对照组(A0)、NaCl胁迫组(A1)、ABA浸种对照组(B0)以及ABA+NaCl胁迫组(B1)中各种色素的含量,发现B1组幼苗的色素含量最高,而A1组幼苗的色素含量最低。在NaCl(150 mmol/L)胁迫条件下,A1、B1中脯氨酸含量分别增加了4.4倍、4.28倍;而在非ABA浸种的对照组(B0)中,脯氨酸含量是对照组(A0)的2.23倍,这说明即使在无盐胁迫条件下,ABA浸种可以同样刺激幼苗中脯氨酸合成、积累。SOD、POD、CAT3种酶活性结果显示,POD活性占主要优势地位,其活性最高的出现在B1组,活力最低的出现在A1组;同对照组(A0)相比,其他3种处理方式使得幼苗中CAT活性明显升高(P〈0.05),而SOD活性明显降低(P〈0.05);但是这3组间CAT、SOD活性差异不明显(P〉0.05)。上述实验结果说明,ABA的预浸种可以提高脯氨酸含量、调节抗氧化物酶的活性,从而维持胁迫条件下植物光合能力,缓解盐胁迫的伤害。

英文摘要:

Pretreatment in plants is recognized as a valuable strategy to eliminate the effects of salt stress. The study evaluated the effects of ABA pretreatment on the photosynthetic pigments, proline concentration, and the antioxidative mechanisms of sunflower under 150 mmol/L NaCl stress. It was found that the concentrations of chlorophyll a, chlorophyll b, carotenoid and total pigments were reduced by 7.8%, 3.6%, 0.5%, 10.9%,respectively. All kinds of pigments concentrations were increased after using ABA pretreatment. Compared within 4 treatments, the most abundant pigment was from the seedlings of B1, while the least pigment was from the seedlings of A1. The concentrations of proline were increased by 4.4 times (A1) and 4.28 times (B1) under salt stress, while the proline concentration of B0treatment was increased by 2.23 times. The result showed that pretreatment with ABA stimulated the accumulation of proline under the control condition (A0). Analysis of the antioxidative enzymes CAT, POD, SOD revealed that ABA pretreatment affected antioxidant enzyme activities,especially POD activity. The most abundant activity was from the seedlings of B1, while the least activity was from the seedlings of A1. Compared with the control, the CAT activity was increased significantly (P0.05),while the SOD activity was decreased significantly (P0.05). The results suggested that ABA pretreatment enhanced the proline concentration, regulated the activities of antioxidants, and maintained the photosynthetic capacity of sunflower seedlings under the salt stress.

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期刊信息
  • 《中国农学通报》
  • 北大核心期刊(2008版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国农学会
  • 主编:石元春
  • 地址:北京市朝阳区麦子店街22号中国农学期刊处
  • 邮编:100025
  • 邮箱:edit@agri.org.cn
  • 电话:010-59194480
  • 国际标准刊号:ISSN:1000-6850
  • 国内统一刊号:ISSN:11-1984/S
  • 邮发代号:2-772
  • 获奖情况:
  • 全国农业核心期刊,中国科协优秀学术期刊,全国农业学会优秀期刊
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2008版)
  • 被引量:94571