中文摘要：

于葡萄黑痘病发病盛期,用病叶压片法对高抗黑痘病的中国野生毛葡萄商-24接种黑痘病病原菌,采用mRNA差异显示技术进行抗黑痘病基因表达差异的研究.结果显示：（1）获得了T11GG/B0304-400、T11CC/S428-350、T11GG/S424-700、T11AG/S432-350、T11GG/S433-250、T11GG/S433-300、T11AG/S432-300、T11GG/S438-353和T11AG/S424-300等9个基因表达差异cDNA片段.其中T11GG/S438-353 mRNA片段表达在接种后3 d被诱导显著降低,并在之后2 d几乎检测不到.（2）采用RACE技术克隆了T11GG/S438-353 mRNA片段的cDNA全长序列;序列分析表明,该cDNA包含一个607 bp完整的开放阅读框架,编码149个氨基酸;其编码氨基酸序列与拟南芥、欧洲葡萄、柳杉、党参、无梗花栎、欧洲栗及寄生草钙调蛋白的一致性分别为99%、97%、94%、91%、90%、88%和77%.（3）本实验克隆到了负向调控中国野生毛葡萄抗黑痘病的钙调蛋白基因,并命名为VqCaM,其GenBank登录号为EU694099;实时荧光定量PCR结果再次验证VqCaM表达受葡萄黑痘病侵染下调,

英文摘要：

mRNA differential display was employed to study the differential expression genes of anthracnose disease resistance with E.ampelina inoculated leaves in Chinese wild V.quinquangularis clone ＇Shang-24＇.Nine differential expressed cDNA fragments T11GG/B0304-400,T11CC/S428-350,T11GG/S424-700,T11AG/S432-350,T11GG/S433-250,T11GG/S433-300,T11AG/S432-300,T11GG/S438-353 and T11AG/S424-300 were obtained,in which a cDNA fragment,T11GG/S438-353,was obtained which expression levels were down-regulated significantly in inoculated leaves during first three days post-inoculation and were nearly undetectable within additional two days.Rapid Amplification of cDNA Ends（RACE） was used to obtain the full-length cDNA sequence of T11GG/S438-353.Full size of 607 bp is obtained and predicted to encode a polypeptide of 149 residues.The deduced amino acid（aa） sequence shared fine identity of 99%,97%,94%,91%,90%,88% and 77% with calmodulin（CaM） proteins from Arabidopsis thaliana,V.vinifera,Cryptomeria japonica,Codonopsis lanceolata,Quercus petraea,Castanea sativa and Striga asiatica respectively.We designated it as VqCaM（GenBank accession number is EU694099）.qRT-PCR result also confirmed the expression pattern of VqCaM post E.ampelina infection in V.quinquangularis ＇Shang-24＇.These results suggest that VqCaM is most likely a specific CaM gene function negatively in message transfer in disease resistance of V.quinquangularis ＇Shang-24＇ against E.ampelina.