中文摘要：

目的研究鸡马立克病病毒（MDV）meq基因对鸡胚成纤维细胞（CEF）的端粒酶催化亚单位基因（chTERT）mRNA转录水平的影响，并探讨meq基因与端粒酶活性的关系。方法构建重组载体pcDNA-meq，转染CEF细胞，利用Taq-Man探针，经实时荧光定量RT-PCR检测meq基因对CEF细胞chTERTmRNA转录水平的影响，并用TRAP法测定端粒酶活性。结果meq基因能激活CEF细胞中chTERTmRNA的转录，在48h的转录水平是72h的16倍，端粒酶活性在转染后48h是转染后72h的12倍。结论meq基因可能是MDV基因组中的主要致瘤基因，可以在体外导入正常的CEF细胞中，激活端粒酶的活性。

英文摘要：

Objective To study the effect of meq gene of Marek disease virus （MDV） on the transcription of chicken telomer- ase reverse transcriptase （chTERT） mRNA in chicken embryofibroblast （CEF） cells and explore the relationship between meq gene and telomerase activity, Methods Construct recombinant plasmid pcDNA-meq for transfection of CEF cells. Determine the transcrip- tion level of chTERT mRNA in CEF cells by real-time fluorescent quantitative RT-PCR using TaqMan probe, and the telomerase activi- ty by TRAP method. Results meq gene activated the transcription of chTERT mRNA in CEF cells. The transcription level 48 h was 16 times higher than that 72 h after transfection. The telomerase activity 48 h was 12 times higher than that 72 h after transfection, Conclusion meq gene might be the main tumorigenic gene in MDV genome. It could be introduced to normal CEF cells in vitro and activate telomerase activity.