中文摘要：

目的：观察刀豆蛋白（ConA）诱导转基因小鼠慢性免疫性肝损伤肝组织细胞免疫功能情况,探讨清利活血健脾中药复方荣肝合剂对其的影响。方法：选用转基因小鼠62只,随机分为模型组、正常组、联苯双酯（DDB）组、茵陈组、茵陈蒿汤组、荣肝合剂组,除正常组外,其余5组采用ConA尾静脉注射的方法制备慢性肝损伤模型。各组均于开始造模的同时给予相应的药物或0.9%氯化钠溶液灌胃,每天1次,连续4周。末次给药后24h,取肝组织光镜下观察其病理变化,流式细胞仪检测CD4＋、CD8＋T细胞的表达情况。结果：病理组织学变化,与正常组比较,模型组肝组织结构被破坏,肝细胞变性坏死,成纤维细胞浸润,大量纤维组织增生,部分肝组织有假小叶形成。各脏器重量指数变化,各治疗组与模型组比较,肝脏指数均有显著性差异（P〈0.05）,其中荣肝合剂组、茵陈蒿汤组脾脏、胸腺指数也有明显变化（P〈0.05）。细胞免疫功能变化,与正常组比较,模型组CD4＋所占比例显著性下降,CD＋8比例显著性升高（P〈0.05,P〈0.01）,药物治疗后,CD4＋/CD8＋的比例有所上升（P〈0.05）,其中荣肝合剂组优于其他治疗组（P〈0.05）。结论：中药复方对慢性免疫性肝损伤的病理损害有明显减轻的作用,其作用的一个方面可能与细胞免疫功能的调节密切相关。

英文摘要：

Objective： To observe the effects of Ronggan Mixture（RGM） on cellular immune function in transgenic mice with chronic Iiver immune injury induced by concanavalin-A（ConA）.Methods： Sixty-two transgenic mice were divided into 6 groups,model group,normal group,and treated group treated respectively with biphenyldimethylesterate（DDB）,oriental wormwood（OWW）,Yinehenhao Decoction（YCHD） and RGM.Pathologic changes of liver tissue were observed by light microscopy,cellular immune function of liver tissues were detected by flow cytometry.Result： Histopathological changes： evident pathological changes of liver appeared in the model mice showed severely destroyed structure of hepatic lobules.As compared with the model group,the changes of liver fibrosis and cell necrosis were much lessened in the RGM group and the YCHD group（P〈0.05）.Index of organ weight changes： compared with model group,index of spleen and thymus increased significantly in YCHD group and Rongganheji group（P〈0.05）.Cellular immune function changes： compared with the normal group,CD4＋ T cells were lower significantly in model group,CD8＋ T cells were higher significantly in model group（P〈0.05,P〈0.01）;the ratio of CD4＋ T and CD8＋ T cells was higher after treating（P〈0.05）,the effect of RGM group was the most obvious（P〈0.05）.Conclusion： RGM may not only have an effect on hepatic pathological injury,but also can improve cellular immune function through regulating the level of CD4＋ and CD8＋ T cells.