中文摘要：

好氧甲烷氧化菌是以甲烷为碳源和能源的细菌。好氧甲烷氧化菌在自然环境中分布广泛，人类已从土壤、淡水和海洋沉积物、泥炭沼泽、热泉、海水和南极环境分离到甲烷氧化菌的纯培养。好氧甲烷氧化菌可分为14个属，包括研究较为深入的隶属于变形菌门Alpha和Gamma纲的细菌，以及属于疣微菌门的极端嗜热嗜酸甲烷氧化菌。最近，好氧甲烷氧化菌还被发现存在于苔藓类植物（尤其是泥炭苔藓）共生体中，兼性营养好氧甲烷氧化菌也被发现。通过对好氧甲烷氧化菌的分类、生理生化特征、分子生物学检测方法以及微生物生态学中的研究成果的总结与分析，以及对甲烷氧化菌研究所面临的问题进行讨论，以期为今后进一步开展好氧甲烷氧化菌及其在碳循环中的作用研究提供参考。

英文摘要：

Aerobic methane oxidizing bacteria （methanotrophs） are a fascinating group of bacteria that have the unique ability to grow on methane as their sole carbon and energy source. They appear to be widespread in nature and have been isolated from a number of different environments. There are now 14 recognized genera of methanotrophs belong to two phyla, Proteobacteria and thermoacidiphilic Verrucomicrobia.The former was well studied and separated into two classes, TypeⅠ and TypeⅡ methanotrophs, which belong to Alpha and Gamma Proteobacteria. Extremely thermophilic, acidophilic methanotrophs from the phylum Verrucomicrobia have been isolated, thus expanding both the taxonomic diversity and physiological range of aerobic methanotrophy. The discovery of the facultative methanotroph Methylocella silvestris has changed the view that methanotrophs were obligate organism. They can cooxidize a considerable number of organic compounds and also have considerable potential in biotechnology. A wide variety of methanotrophic symbionts in and on the mosses were recently detected, and showing the global prevalence of this symbiosis. Traditional way used cultivation to enrichment or isolation to study methanotrophs in the environment. Molecular ecology techniques applied in the last few decades have greatly expanded our knowledge of methanotroph ecology. The most obvious marker for detecting methanotrophs in various environments is the 16S rRNA gene, due to the large database of sequences available. Primers and probes targeting different genera or species have been designed and used extensively in combination with polymerase chain reaction （PCR） based clone library analysis, denaturing gradient gel electrophoresis （DGGE） analysis, and fluorescent in situ hybridization （FISH） analysis. Several functional genes have also been used for the detection of methanotrophs in environmental samples, including pmoA （encoding the key subunits of particulate methane monooxygenase）, mmoX （encoding the key subunits of solu