中文摘要：

背景：正畸牙齿移动过程早期,通过监测龈沟液中相关炎症因子的动态变化水平将有助于评估正畸早期效果,髓过氧化物酶、可溶性细胞间黏附分子1、正五聚蛋白3被证实与炎症密切相关,但当前对于正畸牙齿移动过程中的3种炎症因子的水平以及3种因子水平与正畸牙齿关系还不是十分清楚。目的：通过检测上颌尖牙远中移动过程中不同时间段内龈沟液中髓过氧化物酶、可溶性细胞间黏附分子1、正五聚蛋白3的浓度,了解三者在在正畸治疗过程中的动态变化,探讨其与牙周炎症、尖牙移动距离以及正畸力间相关性。方法：收集21例口腔正畸患者,按照正畸力值分为150 g组（n=12）和100 g组（n=9）,分别加载150 g和100 g拉力。2组患者分别于牙齿正畸受力前、受力后4,12,24 h及7,14 d收集龈沟液,用ELISA试剂盒检测龈沟液内髓过氧化物酶,可溶性细胞间黏附分子1与正五聚蛋白3水平,分析三者在正畸牙齿移动早期的表达变化。结果与结论：在尖牙远中移动早期,患者龈沟液中髓过氧化物酶的表达水平在正畸加力后显著上升,并于4 h达到峰值,后开始下降;而可溶性细胞间黏附分子1在正畸后12 h达到高峰后开始下降,二者在正畸力作用7 d后均恢复正常水平;正五聚蛋白3的表达水平在正畸加力后显著上升,于24 h达到峰值,后开始逐渐下降,同样于正畸治疗后7 d恢复正常水平;此外,加载150 g拉力的患者龈沟液中的髓过氧化物酶,可溶性细胞间黏附分子1与正五聚蛋白3的质量浓度显著高于加载100 g拉力的患者。提示在正畸牙齿移动早期过程中髓过氧化物酶、可溶性细胞间黏附分子1、正五聚蛋白3在龈沟液中呈现动态变化,三者可以有效的反映正畸力作用下牙周组织的炎症反应状况,对其水平的检测将有助于判断正畸力作用效果,预防不良反应发生。

英文摘要：

BACKGROUND： To dynamically monitor the varying levels of inflammatory factors in the gingival crevicular fluid is helpful to assess the early effect of orthodontic tooth movement. Myeloperoxidase, soluble intercellular adhesion molecule-1, pentraxin 3 are proven to be closely related to inflammation, but it is unclear about the levels of these three kinds of inflammatory factors as well as association of these three kinds of inflammatory factors with orthodontic tooth. OBJECTIVE： To detect the expression levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid during maxillary canine distal movement and to assess their correlation with periodontal disease, canine movement distance and orthodontic force. METHODS： Twenty-one orthodontic patients were enrolled and assigned into 150 g（n=12） or 100 g（n=9） groups according to orthodontic force. The gingival crevicular fluid samples of orthodontic patients were collected before and at 4, 12, 24 hours, 7, 14 days after maxillary canine distal movement. Levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid were measured and analyzed using ELISA assay. RESULTS AND CONCLUSION： During the distal movement of maxillary canine, under orthodontic force, the level of myeloperoxidase was peaked at 4 hours and then decreased, while the expression level of soluble intercellular adhesion molecule-1 was peaked at 12 hours, and then decreased. Both myeloperoxidase and soluble intercellular adhesion molecule-1 levels returned to normal at 7 days under orthodontic force. The expression level of pentraxin-3 was increased significantly under orthodontic force, peaked at 24 hours, and then decreased gradually to the normal level at 7 days. In addition, the expression levels of myeloperoxidase, soluble intercellular adhesion molecule-1 and pentraxin-3 in the gingival crevicular fluid were significantly higher under 150 g force than under 100 g force. Th