中文摘要：

为寻找人参病原真菌的生防菌株，采用传统的分离纯化方法，从人参新鲜根中分离内生真菌，并通过平板对峙法筛选高效广谱的菌株；利用形态学与18SrDNA序列分析相结合的手段对菌株进行鉴定．结果表明：从3年生人参新鲜根中分离得到86株内生真菌，其中，Ns-3、Ns-16及Ns-38菌株对人参7种病原菌均有高效广谱抑菌作用，Ns-3对人参根腐病菌的抑茵率达到63．33％，Ns-16对人参灰霉病菌的抑菌率达到69．63％，Ns-38对人参锈腐病菌的抑菌率达到62，34％．通过形态学与18SrDNA序列分析，Ns-3、Ns-38为半知菌亚门青霉属真菌（EpicoccumSP．），Ns-16为半知菌亚门附球菌属黑附球菌（Epicoccumnigrum）．为人参内生生防制剂的开发和利用提供了依据，也为开发经济环保的生物农药提供了菌种资源．

英文摘要：

In order to find biocontrol strains of seven pathogens of Panax ginseng, endophytic fungi were isolated from fresh root of Panax ginseng by traditional methods of separation and purification. The high-efficiency and wide-spectrum antagonistic endophytes were screened using dual culture on potato dextrose agar. The strains were identified by a method which performed by the morphological and 18S rDNA sequence analysis. It was showed that 86 strains endophytic fungi were isolated from 3- year-old fresh root of Panax ginseng. Three of them named Ns-3,Ns-16 and Ns-38 are highly effective broad spectrum antimicrobial effect to seven kinds of pathogenic strains of Panax ginseng. The percentage of bacteriostasis of Ns-3 was reached 63.3% to Fusariurn solani of Panax ginseng. The percentage of bacteriostasis of Ns-16 was reached 69.63% to Botrytis cinerea of Panax ginseng. The percentage of bacteriostasis of Ns-38 was reached 62.34% to Cylindrocarpon destructans of Panax ginseng. The results of morphological and 18S ribosomal DNA sequence analysis showed that Ns-3, Ns-38 were Epicoccum SP.. Ns-16 were Epicoccurn nigrum. The study provide the basis and strains resources, for which development and use of endophytic biocontrol agents of Panax ginseng and economic and environmentally friendly bio-pesticides.