中文摘要：

目的探讨氟中毒大鼠膝关节滑膜中骨形态发生蛋白-2（BMP-2）和骨形态发生蛋白-7（BMP-7）的表达及二者与氟中毒性关节病发病机制的关系。方法断乳2周的SD大鼠32只，按体质量随机分为4组（对照组、低氟组、中氟组和高氟组），每组8只。对照组食用正常饲料；低、中、高氟组分别食用含25％、35％、68％贵州省氟中毒病区玉米（含氟量为148．00mg／kg）的配方饲料，复制燃煤型氟中毒大鼠模型。染氟第140天，用免疫组织化学法检测各组大鼠滑膜组织中BMP-2和BMP-7蛋白的表达，图像分析系统分析吸光度值：HE染色观察各组滑膜组织的形态变化，根据病理分级标准，计算各组滑膜病理学总积分。结果对照组大鼠滑膜组织中BMP-2（32．50±2．73）和BMP-7（38．90±2．56）蛋白少量散在表达；与对照组比较，低、中、高氟组大鼠滑膜组织中BMP-2（59．43±5．12、79．82±6．41、101．76±7．56）和BMP-7（55．10±4．82、78．42±5．61、98．46±6．05）蛋白表达增加（P〈0．05），且随染氟剂量的上升而明显增加（P〈0．05）。滑膜病理学总积分，低、中、高氟组（1．04±0．98、4．69±1．28、8．60±2．07）较对照组（0．54±0．21）显著升高（P〈0．05）。BMP-2和BMP-7蛋白表达与滑膜病理学总积分呈高度正相关（r值分别为0．98、0．99．P〈0．05）。结论BMP-2和BMP-7在氟中毒性关节病的发生发展中具有重要的作用，可能是通过调控成骨过程而发挥作用。

英文摘要：

Objective To observe the expressions of bone morphogenetic protein-2（BMP-2） and bone morphogenetic protein-7 （BMP-7） in the synovial tissue of iluorosis rats and its correlation with pathogenic mechanism of fluorosis arthritis. Methods Thirty-two SD rats were randomly divided into 4 groups： the control group, low, moderate and high-dose fluoride group. The control group ate common fodder. The low, moderate and high dose fluoride group were fed with fodder composed of 25%, 35% and 68% of corn（containing fluorine of 148.00 mg/kg） in chronic endemic fluorosis region in Guizhou Province. After 140 days, the expressions of BMP-2 and BMP-7 protein were determined by immunohistochemistry and assayed the absorbance by computer image-pattern analysis system. Light microscope was used to observe the synovial tissue by Hematoxin Eosin, and calculated the pathological integral of synovium according to pathological grade standard. Results The expressions of BMP-2 （32.50 ± 2.73） and BMP-7（38.90 ± 2.56） in the control group was spare. Compared with the control group, the expressions of BMP-2（59.43 ± 5.12,79.82 ± 6.41,101.76 ± 7.56） and BMP-7（55.10 ± 4.82,78.42 ± 5.61,98.46 ± 6.05） in the synovial tissue was up-regulated in each experimental groups （P 〈 0.05 ）, especially in the moderate- dose and the high-dose groups （P 〈 0.05）. Compared with the control group （0.54 ± 0.21 ）, the pathological integral of synovium inereased（P 〈 0.05） in each experimental groups （1.04 ± 0.98,4.69 ± 1.28,8.60 ± 2.07）. The expressions of BMP-2 and BMP-7 in the synovia] tissue was found to be positively related with the pathological integral of synovium（r = 0.98,0.99, P 〈 0.05）. Conclusion The BMP-2 and BMP-7 play an important role in the development of fluorosis arthritis, probably by affecting osteogenesis.