采用紫外法和GC-MS,研究了柴油污染情况下(10g·L^-1)外生菌根真菌铆钉菇和双色蜡蘑的生长特点及对柴油的降解效率.结果表明:铆钉菇和双色蜡蘑均能利用柴油作为唯一碳源生长.经过14d培养,在柴油含量为10g·L^-1液体培养基内,铆钉菇和双色蜡蘑的质量降解率分别为31.32%和39.90%,且主要减少的是16~21碳烃.不同培养基对外生菌根真菌的降解能力也有显著影响.虽然外生菌根真菌在含有葡萄糖的培养基上的生物量是对照(未添加葡萄糖)的2~5倍,但其对柴油的降解能力明显受到抑制.高浓度的无机氮对外生菌根真菌的降解效率有一定的提高作用,但效果不明显.
The study on the growth characteristics of two ectomycorrhizal fungi Gomphidius viscidus and Laccaria bicolor under 10 g·L-1 of diesel and their degradation effect on the diesel showed that both of the ectomycorrhizal fungi could utilize diesel as their only carbon source.After 14-day culture,the degradation effect was 39.90% for Laccaria bicolor and 31.32% for Gomphidius viscidus,and the degraded hydrocarbons were mainly 16C-22C alkenes.The capability of the ectomycorrhizal fungi to degrade diesel varied with the components of culture medium.When glucose was used as the second carbon source,the biodegradation capability of the fungi was inhibited,though the fungal biomass was 2-5 times as much as that without glucose addition.High concentration of inorganic nitrogen could promote the degradation effect to a certain extent,but not greatly.