中文摘要：

利用本实验室分离保存的猪源副黏病毒JL-1株来提取病毒基因组RNA。参考GenBank发表的相关禽副粘病毒Ⅰ型（Avian paramyxovirus serotype1，APMV-1）基因组序列，设计了8对特异性引物，RT—PCR法分别特异性的扩增出病毒各基因片段，并将各目的基因片段回收纯化，将纯化后的PCR产物进行测序。测定结果得到NP、P、M、F、HN、La、Lb、Lc8段基因序列，利用DNAMAN软件进行拼接得到基因组全长cDNA序列（GenBank登录号：EU546165）。序列分析结果表明，PPMV JL—1株与各地代表株核苷酸同源性87．85％～99．78％，与鹅源新城疫（GPMV）ZJ-1、NA-1株核苷酸同源性分别为83．31％、83．46％，同时根据各毒株F基因开放阅读框前389个核苷酸序列绘制出系统发育进化树，结果表明PPMV JL-1株与LaSota同属于基因Ⅱ型，不同于基因Ⅶ型的ZJ-1和NA-1株的鹅源毒株。

英文摘要：

Porcine paramyxovirus（PPMV）strain JL-1 was separated and conserved by this laboratory. Using the DNAMAN,eight pairs of over lapping primers, which cover the whole genome of avian paramyxovirus serotype 1 （APMV-1） strain,were designed according to the nucleotide sequence reported. Eight over-lapping fragments were amplified by the RT-PCR. The complete genome after sequencing and splicing was logged in the GenBank（Number： EU546165）. Sequence analysis results indicated that the homology of complete genome nucleotide sequence from NDV strains was between 87. 85% and 99. 78% by compared with representative strains from every local. Meanwhile,on the basis of the nucleotide sequence of F gene from each NDV strains in a 389 bp region, the phylogenetic tree was drew,that indicated porcine paramyxovirus virulent strain JL-1 belonged to genotype Ⅱ which was the same with virulent strain Lassota, but different from the traditional low virulent strain Lassota which belonged to genotype Ⅱ ,and goose paramyxovirus strains ZJ-1 and NA-1 ,which belonged to genotype Ⅶ.