中文摘要：

首次结合高温碱裂解和乙二醇体系反应液的优势,对茚三酮定量蛋白质的方法进行了改进,同时对测量过程中的关键参数进行了优化,确定了测量波长为570nm、裂解时间为15min、显色时间为15min和冷却时间为5min.在该改进方法条件下,以BSA（牛血清白蛋白）作为标准样品绘制了标准曲线,验证了该方法具有良好的重复性和稳定性.将该方法与茚三酮直接测定法和考马斯亮蓝染色法进行比较,结果表明其具有更高的灵敏度和准确性,在生物、化学、医学等领域具有广泛应用前景.

英文摘要：

Alkaline hydrolyzed proteins in high temperature and ninhydrin reagent prepared in the ethylene glycol dissolving system were first combined with respective advantage.Several key parameters including measure wavelength,hydrolyzing time,colouration time and cooling time were optimized,which were 570 nm,15 min,15 min and 5 min,respectively.The high repeatability and stability of this Improved Ninhydrin（IN）assay was testified by the standard curve of BSA（Bovine Serum Albumin）.Futhermore,it reveals that IN assay shows higher linear and sensitivity than Direct Ninhydrin and Coomassie Brilliant Blue assay.Therefore,the IN assay has great application prospects in the field of biology,chemistry and medicine owing to its sensitivity,accuracy and stability for determination of a variety of proteins.