中文摘要：

本研究旨在研究不同浓度胰岛素样生长因子I（IG F-I）和表皮生长因子（EGF）对牦牛乳腺上皮细胞SDN1（金黄色葡萄球菌核酸样都铎域1）表达的影响。选取纯化的第3代牦牛乳腺上皮细胞,分别加入0、50、100、200 ng/m L IGF-I或EGF,通过实时荧光定量PCR和免疫荧光检测它们对SND1基因表达及表达蛋白分布的影响。实时荧光定量PCR和免疫荧光分析显示：IGF-I可提高牦牛乳腺上皮细胞SND1基因的表达,且具有浓度依赖性,当IGF-I浓度为200 ng/m L时,SND1基因的白表达量最高,显著高于其他浓度组（P〈0.05）;EGF也可提高SND1基因的表达,当EGF浓度为100 ng/m L时,SND1基因和的表达量最高,显著高于其他浓度组（P〈0.05）。SND1蛋白主要分布于牦牛乳腺上皮细胞胞核中。研究结果表明,IGF-I、EGF可通过调控牦牛乳腺上皮细胞SND1的表达来调节泌乳机能。

英文摘要：

The objective of this study was to detect the effect of different concentrations of IGF-I and EGF on SNDI （Staphylococcal nuclease and tudor domain containing 1）expression of yak mammary epithelial cells. The yak purified mammary epithelial cells in third generation were cultured with the supplement at the concentrations of 0,50,100 and 200 ng/mLof IGF-I or EGF. The gene expression of SNDI was detected by RT-qPCR and its protein level was tested by immunofluorescence. The RT-qPCR and immunofluorescence analysis showed that SNDI gene and protein level were increased by IGF-I, at dose dependent. The expression of SND1 gene and protein was at the highest level with the co-incubation of IGF-I at 200 ng/mL, which was significantly higher than 0, 50, 200 ng/mL teams （P〈0.05）.EGF also up-regulated the expression of SNDI gene and protein, the expression of SND1 gene and protein was at the highest level,when EGF was at 100 ng/mL, which was significantly higher than 0, 50,200 ng/mL teams（P〈 0.05）.The SNDI was mainly distributed in nuclear. It is concluded that IGF-I and EGF regulated the function of mammary epithelial cells of yak through regulating SNDI expression.