中文摘要：

目的：探讨大鼠鞘内注射阿米洛利、吗啡以及两者混合的抗伤害作用。方法：SD大鼠鞘内埋入导管并留置,经导管分别将阿米洛利、吗啡、阿米洛利混合吗啡注入鞘内。以甩尾反应阈值为指标,观察注药前后的抗伤害作用。结果：大鼠鞘内单独注射吗啡（0.25～10μg）和阿米洛利（25～100μg）产生剂量依赖性的抗伤害作用。大鼠鞘内注射阿米洛利（50μg）增强吗啡（1μg、2μg）的抗伤害作用。鞘内预注射纳洛酮具有拮抗吗啡及吗啡混合阿米洛利的抗伤害作用。结论：大鼠鞘内注射阿米洛利存在抗伤害作用,能增强吗啡的抗伤害作用,其作用能被纳洛酮所拮抗。

英文摘要：

Objective： To investigate the antinociceptive effects of intrathecally administrated amiloride and examine its combination effect with spinal morphine. Methods： Male Sprague-Dawley rats were chronically implanted with lumbar intrathecal catheters. The ability of intrathecally administered amiloride and morphine and mixture of amiloride-morphine to alter tail-flick latency was examined. Results： Intrathecally administered amiloride （25 - 100 μg） and morphine （0.25 - 10 μg） produced significant antinociception in a dose-dependent manner. Amiloride （50 μg） enhanced the antinociception of morphine （1 μg,2 μg）. Intrathecally administered naloxone blocked the antinociceptive effect of morphine and morphine-amiloride. Conclusion： Intrathecally administered amiloride produces antinociception and potentiates the action of morphine. Those actions can be blocked by intrathecally administered naloxone.