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古代淀粉鉴别的新方法-耐热性α淀粉酶降解法
  • ISSN号:1001-7410
  • 期刊名称:《第四纪研究》
  • 时间:0
  • 分类:K87[历史地理—考古学及博物馆学;历史地理—历史学] Q94[生物学—植物学]
  • 作者机构:[1]中国科学院研究生院科技史与科技考古系,北京100049, [2]中国科学院古脊椎动物与古人类研究所人类演化实验室,北京100044, [3]法国国家科研中心里昂Archeorient实验室,里昂69001, [4]西班牙巴塞罗那自治大学史前考古系,巴塞罗那08193
  • 相关基金:中国科学院知识创新工程重要方向性项目(批准号:KZCX2-YW-Q1-04);国家自然科学基金项目(批准号:41002057)共同资助
中文摘要:

目前古代淀粉粒鉴别的两种方法,即显微镜观察法和碘着色测试法,均不能确保鉴别的准确性。针对耐热性α-淀粉酶只对淀粉产生降解的特点,设计出古代淀粉粒鉴别的“耐热性α-淀粉酶降解法”,并运用到考古样品中淀粉粒的分析和鉴定之中。本文详细介绍了耐热性α-淀粉酶降解法的原理和流程,并通过古代淀粉粒的鉴别实验,确定了该方法在确切鉴别考古样品中淀粉粒的实用性和有效性;同时现代大麦和莲藕淀粉粒的降解实验结果表明不同植物来源以及同种植物内部不同粒径的淀粉粒其降解速率存在明显差异,而古代淀粉粒在耐热性α-淀粉酶作用下出现的降解速率差异的现象,或可揭示古代淀粉粒的植物来源,甚至长期埋藏对其产生的影响。

英文摘要:

Starch granules are identified by the stains and the extinction cross(Maltese cross) , wmcn can be seen under the polarized light to date. However, both methods have their defects and ancient starch cannot be identified accurately this way. Considering the thermostable alpha-amylase,which is specifically to degrade starch, it is an effective method to identify ancient starch with this enzyme. Two kinds of modern starches from barley and lotus root were chosen for degradation with the thermostable alpha-amylase. They were both degraded and the rates of degradation of two samples were different. The result may show that the rates of degradation from different plants should be different. Meantime, although most of starch granules from barley and lotus root were degraded completely after twenty minutes and one hundred and twenty minutes heating respectively, there survived some smaller granules from each sample. This phenomenon indicates that the size of starch granule may affect the rate of degradation. Based on the degradation of modern starch,ancient suspicious starch from denta| calculus were a|so degraded with the alpha-amylase to test whether this method is effective. These materials were degraded and the result shows that they were definitely starch granules. It should be noted that there were also some smaller starch granules which were not degraded completely after heating. This result is consistent with the modern starch samples and indicates that the size of starch granule may have some effects on its preservation. Modern and ancient starch degradation experiments show that degradation of ancient starch with the thermostable alpha-amylase is an effective method to identify them. Furthermore,this method may also provide some information on diagenesis and plant origins of ancient starch.

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期刊信息
  • 《第四纪研究》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院地质与地球物理研究所 中国第四纪研究委员会
  • 主编:郭正堂
  • 地址:北京9825信箱
  • 邮编:100029
  • 邮箱:dsj@mail.igcas.ac.cn dsjs@mail.igcas.ac.cn
  • 电话:010-82998119
  • 国际标准刊号:ISSN:1001-7410
  • 国内统一刊号:ISSN:11-2708/P
  • 邮发代号:82-428
  • 获奖情况:
  • 1996年被国务院学位委员会地球物理及地质学科评议...,2001年入中国期刊方阵,2007年获中国科学院出版基金科技期刊排行榜三等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:17826