将生长至8~10片真叶的远志幼苗,用市售遮阴网分组处理,包括自然光照组(CK)、轻度遮阴组(30%自然光照)和重度遮阴组(10%自然光照),于处理后15、30 d和60 d测定苯丙素类成分的含量,60 d时测定生理指标,考察光照强度对远志相关生理指标及苯丙素类成分的影响。结果显示3,6'-二芥子酰基蔗糖(DISS)含量与过氧化物酶(POD)活性显著正相关,提示两者在对抗细胞氧化破坏方面可能具有协同作用。遮阴能提高远志幼苗地上、地下部分DISS含量,这可为远志规范化栽培提供依据。
The seedlings of Polygala tenuifolia Willd, :were under different treatments, including natural light group (CK) ,single shading network group (30% natural light) and double shading network group (10% natural light), and were coUected after 15 d,30 d and 60 d respectively. Related physiological indexes and phcnylpropanoid constituents in P. tenuifolla were then detected to investigate the effects of light intensity on physiological indexes and phenylprepanoid constituents in P. tenuifolia. The results showed that, the activity of peroxidase (POD) were significantly correlated with the content of 3,6'-disinapoyl sucrose (DISS). It indicated that, both of them may had a synergistic effect on fighting a- gainst oxidative damage. Short term shade can increase the content of DISS. This study provided the basis for standard- ized cultivation of P. tenuifolia.