中文摘要：

目的：观察多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用。方法：健康SD大鼠32只，随机分为对照组、多西环素组，每组16只大鼠。建立角膜碱烧伤模型后，多西环素组予3g／L多西环素眼液点眼，对照组予眼液溶媒点眼。分别于碱烧伤后第3，7，14，21d观察计算炎症指数，角膜取材后进行病理切片及炎症细胞计数，行ICAM-1的ELISA检测。结果：多西环素组各时间点结膜充血、角膜水肿较对照组轻，炎症指数均明显低于对照组，差异有统计学意义（P〈0．05）。多西环素组各时间点炎症细胞计数少于对照组（P〈0．05）。多西环素组各时间点大鼠角膜的ICAM-1表达低于对照组（P〈0．05）。结论：多西环素可以抑制碱烧伤大鼠角膜的炎症细胞浸润，其机制可能是抑制ICAM-1的表达。

英文摘要：

AIM： To investigate the inhibited effect of Doxycycline on the infiltration of inflammatory cells in rat alkali burn corneas. METHODS： Corneas of 32 SD rats were injured with lmol/L NaOH, then divided into two groups： control and doxycycline - treated. All agents were administered topically 4 times daily. Slit- lamp microscope was performed and inflammatory index was calculated at 3, 7, 14 and 21 days after injury. Then 4 rats were randomly sacrificed and each cornea was divided into two parts, one for histopathology, the other for ICAM- 1 ELISA assay. RESULTS：In control group, the inflammatory index and the number of inflammatory cell was higher than the doxycycline-treated dramatically at all time points （P〈 0.05）. Compared with control group, corneal ICAM-1 expression decreased significantly in doxycycline-treated group at all time points（P 〈 0.05）. CONCLUSION： Doxycycline may inhibit inflammatory cell infiltration by down-regulating ICAM-1 expression.