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4种提取鹰嘴豆基因组DNA方法的比较
  • 期刊名称:新疆农业大学学报
  • 时间:0
  • 页码:163-173
  • 语言:中文
  • 分类:S529[农业科学—作物学]
  • 作者机构:[1]新疆农业大学农业生物技术实验室,乌鲁木齐830052
  • 相关基金:国家自然科学基金项目(30860152)
  • 相关项目:鹰嘴豆中与干旱相关的NAC家族基因的克隆与功能分析
中文摘要:

采用CTAB法、SDS-CTAB法、SDS法、尿素法4种方法对鹰嘴豆DNA进行提取,并从电泳结果、纯度、得率等方面对其进行比较研究,从而确定鹰嘴豆DNA提取适用流程。所提DNA纯度为:CTAB法〉SDS-CTAB法〉SDS法〉尿素法;所提DNA的浓度(μg/mL)为:SDS-CTAB法〉CTAB法〉SDS法〉尿素法。综合分析,CTAB法是提取鹰嘴可DNA的最佳方法。

英文摘要:

The main object of this paper was to study DNA extracted from chickpea by the 4 methods ineluding CTBA method,SDS-CTAB method,SDS method and urea method and to carry out comparative analysis of the method and to carry out comparative analysis of the eletrophorctic results,purity and enzyme digesting of genome DNA. Thereby,the appropriate flow was determined for extracting DNA from chickpea. The purity of extracted DNA was as following:CTAB method〉SDS-CTAB method〉SDS method〉urea method. The concentration(μg/g) of extracted DNA was as following: SDS-CTAB method〉CTAB method〉SDS method〉urea method. The conclusion was drawn:CTAB method was the best one of extracting DNA from chickpea.

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