中文摘要：

目的：探讨热休克蛋白90（HSP90）是否能够抑制肿瘤坏死因子α（TNF-α）和放线菌酮（CHX）协同诱导的细胞凋亡.方法：采用电穿孔技术建立稳定过表达HSP90的细胞克隆,应用激光共聚焦显微镜和流式细胞仪观察TNF-α/CHX协同诱导的细胞凋亡.结果：在稳定过表达HSP90的小鼠成纤维细胞系NIH3T3中,HSP 90能够抑制TNF-α/CHX协同诱导的细胞凋亡;在TNF-α/CHX协同诱导的细胞凋亡中,HSP 90持续稳定存在12 h以上.结论：HSP 90能够抑制TNF-α/CHX协同诱导的细胞凋亡.

英文摘要：

Objective： To investigate whether heat shock protein （HSP90） could suppress the apoptosis synergistically induced by tumor necrosis factor-α （TNF-α） and cycloheximide （CHX）. Methods： The cell clone with stable HSP90 overexpression was estabfished. The TNF-α/CHX induced apoptosis was detected by using laser confocal microscope and flow cytometry. Results： HSP90 suppressed the TNF-α/CHX induced apoptosis in NIH3T3 cells with stable HSP90 overexpression and existed stably for more than 12 hours. Conclusion： HSP90 could suppress TNF-α/CHX induced apoptosis.