中文摘要：

目的：研究早期自然流产患者绒毛肿瘤转移抑制基因KiSS-1与基质金属蛋白酶（MMP-9、MMP-2）mRNA及蛋白的表达，探讨其与自然流产的关系。方法：采用半定量RT—PCR、免疫印迹法检测30例正常早期妊娠和30例早期自然流产患者绒毛KiSS-1、MMP-9和MMP-2的mRNA及蛋白水平，用明胶酶谱分析法检测上述样本孵育液MMP-9、MMP-2的酶活性。结果：（1）自然流产患者绒毛组织中MMP-9及MMP-2mRNA表达水平低于正常早期妊娠组（P〈0．05），KiSS-1mRNA的表达高于正常早期妊娠组差异有统计学意义（P〈0．05）；（2）Western blotting分析显示自然流产患者绒毛MMP-9、MMP-2蛋白表达低于正常早期妊娠差异有统计学意义（P〈0．05），KiSS-1蛋白表达高于正常早期妊娠差异有统计学意义（P〈0．05）；（3）明胶酶谱分析结果显示，自然流产患者绒毛MMP-9、MMP-2酶活性低于正常早期妊娠者差异有统计学意义（P〈0．05）。结论：自然流产患者绒毛促浸润基因MMP-9、MMP-2mRNA及其蛋白表达水平降低，同时抑制浸润基因KiSS-1mRNA及其蛋白表达增加，造成滋养细胞浸润能力下降，可能与自然流产的发生有关。

英文摘要：

Objective：To investigate the mRNA and protein expression of metastasis suppressor gene KiSS-1, matrix metalloproteinase-9 （MMP-9） and MMP-2 in villi of first trimester spontaneous abortion. Methods： Expression of KISS-1, MMP-9 and MMP-2 mRNA and protein in villi of first trimester spontaneous abortion （ abortion group, n = 30） and normal first trimester preg.nant women（ control group,n = 30） was detected by Semi-quantitative RT-PCR , Western blotting and gelatin zymography. Results： （ 1 ） Expression of MMP-9 and MMP-2 mRNA was significantly lower in villi of abortion group compared with that of control group （P 〈 0. 05 ）. Expression of KiSS-lmRNA increased significantly in villi of abortion group （P 〈 0. 05 ） ; （2） Western blotting analysis demonstrated that expression of MMP-9 and MMP-2 protein in villi of abortion group was significantly lower than that of control group （ P 〈 0. 05 ）. Expression of KiSS-1 protein in villi of abortion group was significantly higher than normal（ P 〈0. 05 ） ; （3） Gelatin zymographic analysis showed enzyme activity of MMP-9 and MMP-2 in harvested villi culture medium of abortion group was significantly lower than that of control group （P 〈 0.05 ）. Conclusion ：This study showed that MMP-9, MMP-2 and KiSS-1 may play an important role in the invasion of human placenta trophoblast. It may correlate with occurrence of first trimester spontaneous abortion.