拟南芥中未知基因At1g14260的表达受到多种非生物胁迫的诱导,特别是在NaCl的诱导下,At1g14260的表达量明显增加.对T-DNA插入突变体at1g14260(salk-118406)的分析表明,敲除了基因At1g14260的拟南芥相比野生型对盐胁迫更加敏感.此外,构建了融合表达载体PBi221-At1g14260-GFP并且成功转入拟南芥原生质体中,在荧光显微镜下观察到融合蛋白定位于原生质体细胞核中.因此,At1g14260可能参与了拟南芥中盐胁迫的过程.
The expression of Arabidopsis unknown gene Atlg142600 was induced by a variety of abiotic stresses. The expression of At1g14260 was significantly increased under the treatment of NaC1. The results suggested that At1g14260 was involved in the process of salt stress response. Analysis of the T- DNA mutant of atlg14260 (salk-118406) demonstrated that knocking out Atlg14260 in Arabidopsis resulted in plants more sensitive to NaC1 than the wild type. In addition, fusion vector PBi221-At1g14260- GFP was constructed and transfected into Arabidopsis protoplast. The observation by fluorescence microscope revealed that the fusion protein localized innucleus of the protoplasts. Therefore, At1g14260 may participate in the process of salt stress in Arabidopsis thaLiana.