中文摘要：

以脱硫菌和甲苯降解菌为研究对象,分别以硫代硫酸钠和甲苯为驯化底物,研究了2类菌种的驯化、筛选、组合及混合菌的降解条件.通过驯化菌源,筛选得到高效脱硫菌4株（Z1、Z2、Z3、B1）,甲苯降解菌3株（J1、J2、J3）,并优化组合2类菌种得到混合菌的最佳组合方式为（Z1＋Z2）-B1-（J1＋J3）,且其菌体密度组合比例为2∶4∶3.同时以SO42-的质量浓度和甲苯降解率为控制指标,采用响应面法的中心组合设计（central composite design,CCD）优化混合菌的降解条件,分析得到混合菌的最优降解条件为：pH 6.6,温度30.0℃,接种量3.0％,甲苯添加量3.0μL,硫代硫酸钠加入量4.0 g/L.验证实验表明,在最优条件下混合菌的甲苯降解率为73.9％,培养液中SO42-的质量浓度为163.9 mg/L,基本与预测结果一致,较优化前有明显提高.

英文摘要：

The paper reports the domestication, screening and combination of the toluene degrading bacteria and desulfurizing-bacteria, investigates the degradation conditions of mixed bacteria, with sodium thiosulfate and toluene as substrate, respectively. Four strains of efficient desulfurizing-bacteria （Z1 , Z2, Z3, and B1 ） and three strains of toluene degradation bacteria （J1, J2, and J3） were obtained by domesticating bacteria sources. Then, the two kinds of strains were combined to obtain the best combination mode for （Z1 ＋Z2） -B1 -（J1 ＋ J3）, and the density ratio of thallus ：2： 4： 3. In addition, The central composite design （CCD） of response surface method （RSM） was used for optimizing the degradation condition of the mixed bacteria, SO42- concentration and toluene degradation rate as control index. The optimum conditions were as follows： pH 6.8, temperature 30.0 ℃, inoculation amount 3.0% , toluene mass 3.0 μL/80 mL, and sodium thiosulfate mass 4.4 g/L. The verified experiment shows that toluene degradation rate can reach 73.9% and SOl- concentration of 163.9 mg/L under the best degradation conditions, which was basically identical to the prediction results, and improved significantly compared with removal performance before optimization.