中文摘要：

目的探讨电针对慢性应激模型大鼠行为学的影响及对海马谷氨酸含量的调节作用。方法采用应激刺激作用于SD大鼠制成慢性应激模型，电针“百会”“印堂”穴，每天1次，每次20min；百优解于每日刺激前1h灌胃给药，共21d。通过开野实验及游泳实验进行行为学检测，运用放免法（RIA）检测下丘脑促肾上腺皮质激素释放因子（CRF）、垂体促肾上腺皮质激素（ACTH）、肾上腺及海马皮质酶（CORT）含量，比色法测定海马内谷氨酸（Glu）含量，与空白组、模型组、百优解组做比较。结果与空白组相比，模型组开野实验的水平运动次数和垂直运动次数明显减少，强迫游泳不动时间明显增加，下丘脑CRF、垂体ACTH、肾上腺及海马CORT、海马内Glu含量明显升高；电针组和百优解组开野实验的运动次数明显增加，强迫游泳不动时间明显减少，下丘脑CRF、垂体ACTH、肾上腺及海马CORT、海马Glu含量降低。结论慢性应激引起大鼠的活动性降低，探究行为减少，下丘脑-垂体-肾上腺（HPA）轴亢进并引起大鼠海马内CORT、Glu含量升高，电针可能通过调整HPA轴相关激素水平纠正海马内CORT、Glu含量，从而改善慢性应激大鼠的行为表现。

英文摘要：

Objective To investigate the effect of electroacupuncture （EA） on changes of behavior and glutamic acid level in hippocampal tissue in rats with chronic stress. Methods Forty male SD rats were evenly randomized into control group, stress- model group, EA group and Prozac220 group. Chronic stress model was established by using mixed stimulations. One hour before the stress stimulation, EA was applied to baihui（GV20） and yintang（ EX-HN1 ） for 20 minutes, once daily; Prozac220 was given by intragastric administration once daily, both for 21 days. The rats＇behavior was observed by using openield method and swim. The contents of corticotropin releasing factor （CRF） in the hypothalamic tissue, adrenocorticotropic hormone （ACTH） in the pituitary, and corticosterone （CORT） in the adrenal tissue and hippocampal tissue were detected by radioimmunoassay. Glutamic acid （Glu） in the hippocampal tissue was detected by electro-chemical detection technique. Results Compared with the control group, the rats horizontal and vertical movement times decreased significantly and swim stop time increased significantly in the model group. The contents of hypothalamic CRF, pituitary ACTH , adrenal and hippocampalCORT and hippocampal Glu increased remarkably in the model group （P 〈 0.05 ） . In comparison with the model group, the rats＇ movement times increased and swim stop time decreased considerably, while CRF , CORT and Glu contents lowered significantly in both EA and Prozac220 groups （P 〈 0. 05 ）. Conclusion Chronic stress may cause the stress rats to move less and result in hyperactivity of the HPA axis and high level of Glu in hippocamp, whereas electroacupuncture can promote recovery of the rats＇abnormal behavior and regulate the secretion of Glu in hippocampal tissue.