To verify the theoretical models of varying transversely isotropicstress-strain relations of dentin established in the preceding work(Part Ⅰ),we per-form a set of experiments.Because of the very fine tooth size,it usually seems tobe difficult to directly measure the inhomogeneous and anisotropic parameters ofdentin.In this paper,by the digital speckle correlation method,tensile experimentsare made on the small dentin samples either parallel or perpendicular to the dentintubules.With the theoretically predicted elastic stress-strain relations,an optimiza-tion method is proposed to fit the strain curve adapted to the experimental data.The results show that the theoretical elastic stress-strain relations coincides very wellwith the experimental observations.The determined Young’s modulus and Poisson’sratio of dentin matrix are 29.5GPa and 0.44,respectively,in the optimization sense.