中文摘要：

目的研究ELAC2在体内外对前列腺癌生长的影响。方法利用脂质体转染法将ELAC2基因转入Du-145细胞,建立稳定表达ELAC2的细胞株,同时设立未转染Du-145和转染空载体的Du-145/pcDNA3作为对照。采用RT-PCR和Western blot法检测转染前后ELAC2的mRNA和蛋白表达水平;MTT法检测细胞生长和增殖情况。采用BALB/c裸鼠建立Du-145前列腺癌移植瘤动物模型,检测在体内ELAC2对前列腺癌生长的影响。结果 RT-PCR检测结果显示,ELAC2在Du-145/ELAC2细胞中mRNA表达明显增加,Western blot法检测同样发现ELAC2蛋白表达水平显著升高。MTT检测结果表明,Du-145/ELAC2细胞增殖较对照组细胞明显减缓（P〈0.05）。在体内,Du-145/ELAC2组平均瘤质量和瘤体积均较对照组明显降低（P〈0.05）。结论提高ELAC2基因表达水平能明显抑制体内外前列腺癌细胞Du-145的生长,提示ELAC2可作为前列腺癌治疗的一个新基因。

英文摘要：

Objective To identify the effect of ELAC2 on the growth of human prostate cancer Du-145 cells in vitro and in vivo.Methods ELAC2 was stable transfected into prostate cancer Du-145 cells by Lipofectamine transfection assay,while Du-145 cells transfected with/without empty vector pcDNA3.0 were also developed as the negative controls.The mRNA and protein expression of ELAC2 were detected by RT-PCR and Western blot assay.Cell proliferation was assessed by MTT assay.The allograft prostate tumor model was developed in BALB/c nude mice,which were subcutaneously inoculated with Du-145/ELAC2 cells and the negative control cells,and was used to detect the effects of ELAC2 on tumor growth in vivo.Results As determinated by RT-PCR and Western blot assay,mRNA and protein expression of ELAC2 were significantly increased in Du-145/ELAC2 cells.Cell proliferation of Du-145/ELAC2 was obviously decreased compared with that of negative control cells（P0.05）.Tumor size of Du-145/ELAC2 was obviously smaller than the control tumor size（P0.05）.Conclusion Overexpression of ELAC2 significantly inhibits proliferation of human prostate cancer Du-145 cells in vitro and in vivo,which suggests that ELAC2 may be a novel target for prostate cancer therapy.