硝化污泥强化培养及投加作为一种传统生物脱氮突发事故的应急手段,具有十分重要的实际意义。该试验通过条件控制对硝化污泥强化培养,并且对其性能进行分析。在氨氮浓度为45 mg/L,当C/N为2.2∶1,p H为8.0,水力停留时间(HRT)27 h时,氨氮去除率稳定在90%以上,实现硝化菌富集。分别在30、40、50、60 mg/L 4个氨氮浓度梯度下进行硝化速率和硝化菌个数分析。结果表明,氨氮浓度为40 mg/L时硝化速率最高,达到0.749 4 mg/(g·h),其对应硝化菌的数量最多,其中亚硝化细菌的个数为2.85×106MPN/g MLSS,硝化细菌的个数为1.35×105MPN/g MLSS。
The cultivation of nitrifying sludge enrichment is an effective method of dealing with the emergency occurring in traditional biological nitrification process. The nitrifying sludge enrichment was carried out and the comprehensive performance was also analyzed. With the influent ammonia concentration of 45 mg/L,the ammonia removal efficiency was obtained above 90% under the optimized conditions of p H 8.0,C/N ratio of 2.2 ∶1,and HRT of 27 h. Meanwhile,the nitrifying rate and the amount of nitrifying bacteria in sludge were measured in different ammonia concentrations of 30,40,50 and 60 mg/L. It was showed that the highest nitrifying rate of 0.749 4 mg/(g·h) was determined at the concentration of 40mg/L,with the amount of ammonia oxidizing bacteria as 2.85×106 MPN/g MLSS and the nitrite oxidizing bacteria as 1.35 ×105MPN/g MLSS respectively.